Summary
Adenoviruses (Ads) possess several features that make them attractive mammalian gene transfer vectors. They can efficiently infect a wide variety of quiescent and proliferating cell types from various species to direct high level viral gene expression, their 36 kb double-stranded DNA genome can be manipulated with relative ease by conventional molecular biology techniques, and they can be readily propagated and purified to yield high titer preparations of very stable virus. Consequently, Ads have been extensively used as vectors for recombinant vaccines, for high-level protein production in cultured cells and for gene therapy where high-level, transient transgene expression is desired. This chapter describes in detail methods for the production and characterization of E1-deleted, first generation adenoviral vectors.
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Acknowledgments
PN is supported by grants from the National Institutes of Health (P50 HL59314, R01 DK067324, R01 HL083047 and P51 RR13986), the Texas Affiliate of the American Heart Association (0465102Y) and the Cystic Fibrosis Foundation (NG0530 and NG05G0).
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Palmer, D.J., Ng, P. (2008). Methods for the Production of First Generation Adenoviral Vectors. In: Gene Therapy Protocols. Methods in Molecular Biology™, vol 433. Humana Press. https://doi.org/10.1007/978-1-59745-237-3_4
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DOI: https://doi.org/10.1007/978-1-59745-237-3_4
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