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Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases

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Chromosomal Mutagenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 435))

Abstract

Zinc-finger nucleases (ZFNs) are promising new tools for enhancing the efficiency of gene targeting in many organisms. Because of the flexibility of zinc finger DNA recognition, ZFNs can be designed to bind many different genomic sequences. The double-strand breaks they create are repaired by cellular processes that generate new mutations at the cleavage site. In addition, the breaks can be repaired by homologous recombination with an exogenous donor DNA, allowing the experimenter to introduce designed sequence alterations. We describe the construction of ZFNs for novel targets and their application to targeted mutagenesis and targeted gene replacement in Drosophila melanogaster and Caenorhabditis elegans.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT

    Dana Carroll, Kelly J. Beumer, J. Jason Morton, Ana Bozas & Jonathan K. Trautman

Authors
  1. Dana Carroll
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  2. Kelly J. Beumer
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  3. J. Jason Morton
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  4. Ana Bozas
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  5. Jonathan K. Trautman
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Editor information

Editors and Affiliations

  1. Sigma-Aldrich Biotechnology, Sigma-Aldrich Corporation, St. Louis, Missouri

    Gregory D. Davis

  2. SAFC Biosciences, Sigma-Aldrich Corporation, St. Louis, Missouri

    Kevin J. Kayser

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© 2008 Humana Press Inc., Totowa, NJ

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Carroll, D., Beumer, K.J., Morton, J.J., Bozas, A., Trautman, J.K. (2008). Gene Targeting in Drosophila and Caenorhabditis elegans With Zinc-Finger Nucleases. In: Davis, G.D., Kayser, K.J. (eds) Chromosomal Mutagenesis. Methods in Molecular Biology, vol 435. Humana Press. https://doi.org/10.1007/978-1-59745-232-8_5

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  • DOI: https://doi.org/10.1007/978-1-59745-232-8_5

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-899-7

  • Online ISBN: 978-1-59745-232-8

  • eBook Packages: Springer Protocols

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