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Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells

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Host-Pathogen Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 470))

Abstract

Phagocytosis is a highly conserved aspect of innate immunity. Drosophila melanogaster has an innate immune system with many similarities to that of mammals and has been used to successfully model many aspects of innate immunity. The recent availability of Ribo Nucleic Acid interference (RNAi) libraries for Drosophila has made it possible to efficiently screen for genes important in aspects of innate immunity. We have screened an RNAi library representing 7216 fly genes conserved among metazoans to identify proteins required for the phagocytosis of the human fungal pathogen Candida albicans.

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References

  1. Elrod-Erickson, M., Mishra, S., and Schneider, D. (2000) Interactions between the cellular and humoral immune responses in Drosophila. Curr Biol 10, 781–784.

    CAS  Google Scholar 

  2. Hoffmann, J. A., Kafatos, F. C., Janeway, C. A., and Ezekowitz, R. A. (1999) Phylogenetic perspectives in innate immunity. Science 284, 1313–1318.

    Article  CAS  PubMed  Google Scholar 

  3. Romani, L. (2004) Immunity to fungal infections. Nat Rev Immunol 4, 1–23.

    Article  PubMed  Google Scholar 

  4. Alarco, A. M., Marcil, A., Chen, J., Suter, B., Thomas, D., and Whiteway, M. (2004) Immune-deficient Drosophila melanogaster: a model for the innate immune response to human fungal pathogens. J Immunol 172, 5622–5628.

    CAS  PubMed  Google Scholar 

  5. Cheng, L. W., and Portnoy, D. A. (2003) Drosophila S2 cells: an alternative infection model for Listeria monocytogenes. Cell Microbiol 5, 875–885.

    Article  CAS  PubMed  Google Scholar 

  6. Dionne, M. S., Ghori, N., and Schneider, D. S. (2003) Drosophila melanogaster is a genetically tractable model host for Mycobacterium marinum. Infect Immun 71, 3540–3550.

    Article  CAS  PubMed  Google Scholar 

  7. Mansfield, B. E., Dionne, M. S., Schneider, D. S., and Freitag, N. E. (2003) Exploration of host-pathogen interactions using Listeria monocytogenes and Drosophila melanogaster. Cell Microbiol 5, 901–911.

    Article  CAS  PubMed  Google Scholar 

  8. Schneider, D., and Shahabuddin, M. (2000) Malaria parasite development in a Drosophila model. Science 288, 2376–2379.

    Article  CAS  PubMed  Google Scholar 

  9. Lavine, M. D., and Strand, M. R. (2002) Insect hemocytes and their role in immunity. Insect Biochem Mol Biol 32, 1295–1309.

    Article  CAS  PubMed  Google Scholar 

  10. Agaisse, H., Burrack, L. S., Philips, J. A., Rubin, E. J., Perrimon, N., and Higgins, D. E. (2005) Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Science 309, 1248–1251.

    Article  CAS  PubMed  Google Scholar 

  11. Philips, J. A., Rubin, E. J., and Perrimon, N. (2005) Drosophila RNAi screen reveals CD36 family member required for mycobacterial infection. Science 309, 1251–1253.

    Article  CAS  PubMed  Google Scholar 

  12. Ramet, M., Manfruelli, P., Pearson, A., Mathey-Prevot, B., and Ezekowitz, R. A. (2002) Functional genomic analysis of phagocytosis and identification of a Drosophila receptor for E. coli. Nature 416, 644–648.

    CAS  Google Scholar 

  13. Stroschein-Stevenson, S. L., Foley, E., O’Farrell, P. H., and Johnson, A. D. (2006) Identification of Drosophila gene products required for phagocytosis of Candida albicans. PLoS Biol 4, e4.

    Article  PubMed  Google Scholar 

  14. Edmond, M. B., Wallace, S. E., McClish, D. K., Pfaller, M. A., Jones, R. N., and Wenzel, R. P. (1999) Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis 29, 239–244.

    Article  CAS  PubMed  Google Scholar 

  15. Calderone, R. (Ed.) (2002) Host recognition by Candida species, ASM Press, ASM Press, Washington, DC.

    Google Scholar 

  16. Foley, E., and O’Farrell, P. H. (2004) Functional dissection of an innate immune response by a genome-wide RNAi screen. PLoS Biol 2, E203.

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA

    Shannon L. Stroschein-Stevenson

  2. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada

    Edan Foley

  3. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA

    Patrick H. O’Farrell

  4. Department of Microbiology and Immunology, and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA

    Alexander D. Johnson

Authors
  1. Shannon L. Stroschein-Stevenson
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  2. Edan Foley
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  3. Patrick H. O’Farrell
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  4. Alexander D. Johnson
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Editor information

Editors and Affiliations

  1. Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, Germany

    Steffen Rupp  & Kai Sohn  & 

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Stroschein-Stevenson, S.L., Foley, E., O’Farrell, P.H., Johnson, A.D. (2009). Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells. In: Rupp, S., Sohn, K. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 470. Humana Press. https://doi.org/10.1007/978-1-59745-204-5_24

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  • DOI: https://doi.org/10.1007/978-1-59745-204-5_24

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-886-7

  • Online ISBN: 978-1-59745-204-5

  • eBook Packages: Springer Protocols

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