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Production of Hepatocyte-Like Cells from Human Amnion

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Hepatocyte Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 481))

Abstract

Cells isolated from the placenta have been the subject of intense investigation because many of the cells express characteristics of multipotent or even pluripotent stem cells. Cells from the placental tissues such as amnion and chorion have been reported to display multilineage differentiation and surface marker and gene expression patterns consistent with embryonic stem (ES) and mesenchymal stem cells, respectively. We have reported that epithelial cells isolated from term placenta contain cells that express surface markers such as the stage-specific embryonic antigens (SSEA) and a gene expression profile that is similar to ES cells. When subjected to specific differentiation protocols, amniotic epithelial cells display markers of differentiation to cardiomyocytes, neurons, pancreatic cells and hepatocytes. If specific and efficient methods could be developed to induce differentiation of these cells to hepatocytes, the amnion may become a useful source of cells for hepatocyte transplants. Cells isolated from amnion also have some unique properties as compared to some other stem cell sources in that they are isolated from a tissue that is normally discarded following birth, they are quite plentiful and easily isolated and they do not produce tumors when transplanted. Cells isolated from the amnion may be a uniquely useful and noncontroversial stem cell source.

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References

  1. Strom, S., Bruzzone, P., Cai, H., et al. (2006) Hepatocyte transplantation: clinical experience and potential for future use. Cell Transpl 15, S105–S10.

    Article  Google Scholar 

  2. Fisher, R. A., Strom, S. C. (2006) Human hepatocyte transplantation: worldwide results. Transplantation 82, 441–9.

    Article  PubMed  Google Scholar 

  3. Nakazawa, F., Cai, H., Miki, T., et al. (2002) in Proceedings of Falk Symposium, Hepatocyte Transplantation, Vol. 126, pp. 147-58, Kouwer Academic Publishers, Lancaster, UK.

    Google Scholar 

  4. Mitry, R. R., Hughes, R. D., Dhawan, A. (2002) Progress in human hepatocytes: isolation, culture & cryopreservation. Semin Cell Dev Biol 13, 463–7.

    Article  PubMed  CAS  Google Scholar 

  5. Strom, S., Fisher, R. (2003) Hepatocyte transplantation: new possibilities for therapy. Gastroenterology 124, 568–71.

    Article  PubMed  Google Scholar 

  6. Miki, T., Lehmann, T., Cai, H., et al. (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23, 1549–59.

    Article  PubMed  CAS  Google Scholar 

  7. Miki, T., Strom, S. C. (2006) Amnion- derived pluripotent/multipotent stem cells. Stem cell reviews 2, 133–42.

    Article  PubMed  CAS  Google Scholar 

  8. Davila, J. C., Cezar, G. G., Thiede, M., et al. (2004) Use and application of stem cells in toxicology. Toxicol Sci 79, 214–23.

    Article  PubMed  CAS  Google Scholar 

  9. Elwan, M. A., Sakuragawa, N. (1997) Evidence for synthesis and release of catecholamines by human amniotic epithelial cells. Neuroreport 8, 3435–8.

    Article  PubMed  CAS  Google Scholar 

  10. Elwan, M. A., Ishii, T., Ono, F., et al. (1999) Evidence for the presence of dopamine D1 receptor mRNA and binding sites in monkey amniotic epithelial cells. Neurosci Lett 262, 9–12.

    Article  PubMed  CAS  Google Scholar 

  11. Elwan, M. A., Ishii, T., Sakuragawa, N. (2003) Characterization of the dopamine transporter gene expression and binding sites in cultured human amniotic epithelial cells. Neurosci Lett 342, 61–4.

    Article  PubMed  CAS  Google Scholar 

  12. Sakuragawa, N., Thangavel, R., Mizuguchi, M., et al. (1996) Expression of markers for both neuronal and glial cells in human amniotic epithelial cells. Neurosci Lett 209, 9–12.

    Article  PubMed  CAS  Google Scholar 

  13. Sakuragawa, N., Misawa, H., Ohsugi, K., et al. (1997) Evidence for active acetylcholine metabolism in human amniotic epithelial cells: applicable to intracerebral allografting for neurologic disease. Neurosci Lett 232, 53–6.

    Article  PubMed  CAS  Google Scholar 

  14. Tamagawa, T., Ishiwata, I., Saito, S. (2004) Establishment and characterization ofa pluripotent stem cell line derived from human amniotic membranes and initiation of germ layers in vitro. Hum Cell 17, 125–30.

    Article  PubMed  Google Scholar 

  15. Ilancheran, S., Michalska, A., Peh, G., et al. (2007) Stem cells derived from human fetal membranes display multi-lineage differentiation potential. BiolReprod 77, 577–588.

    Article  CAS  Google Scholar 

  16. Kakishita, K., Elwan, M. A., Nakao, N., et al. (2000) Human amniotic epithelial cells produce dopamine and survive after implantation into the striatum of a rat model of Parkinson’s disease: a potential source of donor for transplantation therapy. Exp Neurol 165, 27–34.

    Article  PubMed  CAS  Google Scholar 

  17. Kakishita, K., Nakao, N., Sakuragawa, N., et al. (2003) Implantation of human amniotic epithelial cells prevents the degeneration of nigral dopamine neurons in rats with 6- hydroxydopamine lesions. Brain Res 980, 48–56.

    Article  PubMed  CAS  Google Scholar 

  18. Wei, J. P., Zhang, T. S., Kawa, S., et al. (2003) Human amnion-isolated cells normalize blood glucose in streptozotocin- induced diabetic mice. Cell Transplant 12, 545–52.

    PubMed  Google Scholar 

  19. In’t Anker, P. S., Scherjon, S. A., Kleijburg- van der Keur, C., et al. (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22, 1338–45.

    Article  Google Scholar 

  20. In’t Anker, P. S., Scherjon, S. A., Kleijburg- van der Keur, C., et al. (2003) Amniotic fluid as a novel source of mesenchymal stem cells for therapeutic transplantation. Blood 102, 1548–9.

    Article  Google Scholar 

  21. Prusa, A. R., Marton, E., Rosner, M., et al. (2003) Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? HumReprod 18, 1489–93.

    Google Scholar 

  22. Tsai, M. S., Lee, J. L., Chang, Y. J., et al. (2004) Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel twO-stage culture protocol. Hum Reprod 19, 1450–6.

    Article  PubMed  Google Scholar 

  23. Tsai, M. S., Hwang, S. M., Tsai, Y. L., et al. (2006) Clonal amniotic fluid-derived stem cells express characteristics ofboth mesenchymal and neural stem cells. Biol Reprod 74, 545–51.

    Article  PubMed  CAS  Google Scholar 

  24. De Coppi, P., Bartsch G., Jr., Siddiqui, M. M., et al. (2007) Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnol 25,100–6.

    Article  PubMed  CAS  Google Scholar 

  25. De Coppi, P., Callegari, A., Chiavegato, A., et al. (2007) Amniotic fluid and bone marrow derived mesenchymal stem cells can be converted to smooth muscle cells in the cryO- injured rat bladder and prevent compensatory hypertrophy of surviving smooth muscle cells. J Urol 177, 369–76.

    Article  PubMed  Google Scholar 

  26. Parolini, O., Alviano, F., Bagnara, G. P., et al. (2007) CONCISE REVIEW: Isolation and Characterization of Cells from Human Term Placenta: Outcome of the First International Workshop on Placenta Derived Stem Cells. Stem Cells.

    Google Scholar 

  27. Miki, T., Marongiu, F., Ellis, E., et al. (2007) Isolation of amniotic epithelial cells. Current Protocols in Stem Cell Biology 1E.3, 1E.3. 1-1E.3.9.

    Google Scholar 

  28. Alexander, J., Stainier, D. Y. (1999) A molecular pathway leading to endoderm formationin zebrafish. Curr Biol 9, 1147–57.

    Article  PubMed  CAS  Google Scholar 

  29. Zoltewicz, J. S., Gerhart, J. C. (1997) The Spemann organizer of Xenopus is patterned along its anteroposterior axis at the earliest gastrula stage. Dev Biol 192, 482–91.

    Article  PubMed  CAS  Google Scholar 

  30. Woo, K., Fraser, S. E. (1997) Specification of the zebrafish nervous system by nonaxial signals. Science 277, 254–7.

    Article  PubMed  CAS  Google Scholar 

  31. Darras, S., Marikawa, Y., Elinson, R. P., et al. (1997) Animal and vegetal pole cells of early Xenopus embryos respond differently to maternal dorsal determinants: implications for the patterning of the organiser. Development 124, 4275–86.

    PubMed  CAS  Google Scholar 

  32. D’Amour, K. A., Agulnick, A. D., Eliazer, S., et al. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat Biotechnol 23, 1534–41.

    Article  PubMed  Google Scholar 

  33. D’Amour, K. A., Bang, A. G., Eliazer, S., et al. (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24, 1392–401.

    Article  PubMed  Google Scholar 

  34. McLean, A. B., D’Amour, K. A., Jones, K. L., et al. (2007) Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3- kinase signaling is suppressed. Stem Cells 25, 29–38.

    Article  PubMed  CAS  Google Scholar 

  35. Soto-Gutierrez, A., Kobayashi, N., Rivas- Carrillo, J. D., et al. (2006) Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes. Nat Biotechnol 24, 1412–9.

    Article  PubMed  CAS  Google Scholar 

  36. Xiao, L., Yuan,X., Sharkis,S.J. (2006) Activin A maintains self-renewal and regulates fibrO- blast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells. Stem Cells 24,1476–86.

    Article  PubMed  CAS  Google Scholar 

  37. Smith, J. R., Vallier, L., Lupo, G., et al. (2008) Inhibition of Activin/Nodal signaling promotes specification of human embryonic stem cells into neuroectoderm. DevBiol 313, 107–117.

    CAS  Google Scholar 

  38. Asahina, K., Fujimori, H., Shimizu-Saito, K., et al. (2004) Expression of the liver-specific gene Cyp7a1 reveals hepatic differentiation in embryoid bodies derived from mouse embryonic stem cells. Genes Cells 9, 1297–308.

    Article  PubMed  CAS  Google Scholar 

  39. Ellis, E., Goodwin, B., Abrahamsson, A., et al. (1998) Bile acid synthesis in primary cultures of rat and human hepatocytes. Hepatology 27, 615–20.

    Article  PubMed  CAS  Google Scholar 

  40. Ellis, E. C. S. (2003) in Department of Medicine, Karolinska Institute, Stockholm.

    Google Scholar 

  41. Yang, H. Y., Lee, Q. P., Rettie, A. E., et al. (1994) Functional cytochrome P4503A isoforms in human embryonic tissues: expression during organogenesis. Mol Pharmacol 46, 922–8.

    PubMed  CAS  Google Scholar 

  42. Schuetz, J. D., Beach, D. L., Guzelian, P. S. (1994) Selective expression of cytochrome P450 CYP3A mRNAs in embryonic and adult human liver. Pharmacogenetics 4, 11–20.

    Article  PubMed  CAS  Google Scholar 

  43. Wrighton,S.A., Molowa,D. T., Guzelian, P. S. (1988) Identification of a cytochrome P- 450 in human fetal liver related to glucocorticoid-inducible cytochrome P-450HLp in the adult. Biochem Pharmacol 37, 3053–5.

    Article  Google Scholar 

  44. Takashima, S., Ise, H., Zhao, P., et al. (2004) Human amniotic epithelial cells possess hepatocyte-like characteristics and functions. Cell Struct Funct 29, 73–84.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Toshio Miki
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  2. Fabio Marongiu
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  3. Ewa C.S. Ellis
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  4. Ken Dorko
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  5. Keitaro Mitamura
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  6. Aarati Ranade
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  7. Roberto Gramignoli
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  8. Julio Davila
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  9. Stephen C. Strom
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Editor information

Editors and Affiliations

  1. King’s College Hospital, London, UK

    Anil Dhawan

  2. School of Medicine, King’s College London, London, UK

    Robin D. Hughes

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Miki, T. et al. (2009). Production of Hepatocyte-Like Cells from Human Amnion. In: Dhawan, A., Hughes, R. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 481. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-201-4_13

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  • DOI: https://doi.org/10.1007/978-1-59745-201-4_13

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-172-1

  • Online ISBN: 978-1-59745-201-4

  • eBook Packages: Springer Protocols

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