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Human Hepatocyte Transplantation Overview

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Hepatocyte Transplantation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 481))

Abstract

The interest in hepatocyte transplantation has been growing continuously in recent years and this treatment may represent an alternative clinical approach for patients with acute liver failure and liverbased metabolic disorders. This chapter presents an overview of liver cell transplantation, from the basic research to human experience. It summarizes the pre-clinical studies and present status of clinical hepatocyte transplantation and identifies some possible areas of future research in this area.

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References

  1. Pereira, S. P., McCarthy, M., Ellis, A. J., et al. (1997) Auxiliary partial orthotopic liver transplantation for acute liver failure. J Hepatol 26, 1010–1017.

    Article  PubMed  CAS  Google Scholar 

  2. Strom, S. C., Fisher, R. A., Thompson, M. T., et al. (1997) Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure. Transplantation 63, 559–569.

    Article  PubMed  CAS  Google Scholar 

  3. Bilir, B. M., Guinette, D., Karrer, F., et al. (2000) Hepatocyte transplantation in acute liver failure. Liver Transpl 6, 32–40.

    PubMed  CAS  Google Scholar 

  4. Schneider, A., Attaran, M., Meier, P. N., et al. (2006) Hepatocyte transplantation in an acute liver failure due to mushroom poisoning. Transplantation 82, 1115–1116.

    Article  PubMed  Google Scholar 

  5. Fox, I. J., Chowdhury, J. R., Kaufman, S. S., et al. (1998) Treatment of the Crigler-Najjar syndrome type I with hepatocyte transplantation. N Engl J Med 338, 1422–1426.

    Article  PubMed  CAS  Google Scholar 

  6. Ambrosino, G., Varotto, S., Strom, S. C., et al. (2005) Isolated hepatocyte transplantation for Crigler-Najjar syndrome type 1. Cell Transpl 14, 151–157.

    Article  Google Scholar 

  7. Muraca, M., Gerunda, G., Neri, D., et al. (2002) Hepatocyte transplantation as a treatment for glycogen storage disease type 1a. Lancet 359, 317–318.

    Article  PubMed  Google Scholar 

  8. Horslen, S. P., McCowan, T. C., Goertzen, T. C., et al. (2003) Isolated hepatocyte transplantation in an infant with a severe urea cycle disorder. Pediatrics 111, 1262–1267.

    Article  PubMed  Google Scholar 

  9. Stephenne, X., Najimi, M., Sibille, C., et al. (2006) Sustained engraftment and tissue enzyme activity after liver cell transplantation for argininosuccinate lyase deficiency. Gastroenterology 130, 1317–1323.

    Article  PubMed  Google Scholar 

  10. Mitry, R. R., Hughes, R. D., Aw, M. M., et al. (2003) Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transpl 12, 69–74.

    Article  Google Scholar 

  11. Mitry, R. R., Dhawan, A., Hughes, R. D., et al. (2004) One liver, three recipients: segment IV from split-liver procedures as a source of hepatocytes for cell transplantation. Transplantation 77, 1614–1616.

    Article  PubMed  Google Scholar 

  12. Muiesan, P. (2003) Can controlled non- heart-beating donors provide a solution to the organ shortage? Transplantation 75, 1627–1628.

    Article  PubMed  Google Scholar 

  13. Hughes, R. D., Mitry, R. R., Dhawan, A., et al. (2006) Isolation of hepatocytes from livers from non-heart-beating donors for cell transplantation. Liver Transpl 12, 713–717.

    Article  PubMed  Google Scholar 

  14. Kobayashi, N., Fujiwara, T., Westerman, K. A., et al. (2000) Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science 287, 1258–1262.

    Article  PubMed  CAS  Google Scholar 

  15. Cai, J., Ito, M., Nagata, H., et al. (2002) Treatment of liver failure in rats with end stage cirrhosis by transplantation of immortalized hepatocytes. Hepatology 36, 386–394.

    Article  PubMed  CAS  Google Scholar 

  16. Dan, Y. Y., Riehle, K. J., Lazaro, C., et al. (2006) Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proc Natl Acad Sci USA 103, 9912–9917.

    Article  PubMed  CAS  Google Scholar 

  17. Avital, I., Feraresso, C., Aoki, T., etal. (2002) Bone marrow-derived liver stem cell and mature hepatocyte engraftment in livers undergoing rejection. Surgery 132, 384–390.

    Article  PubMed  Google Scholar 

  18. Mild, T., Lehmann, T., Cai, H., et al. (2005) Stem cell characteristics of amniotic epithelial cells. Stem Cells 23, 1549–1559.

    Article  Google Scholar 

  19. Ruhnke, M., Ungefroren, H., Nussler, A., et al. (2005) Differentiation of in vitrO-modified human peripheral blood monocytes into hepatocyte-like and pancreatic islet-like cells. Gastroenterology 128, 1774–1786.

    Article  PubMed  CAS  Google Scholar 

  20. Strom, S. C., Dorko, K., Thompson, M. T., et al. (1998) Large scale isolation and culture of human hepatocytes, in (Franco, D., et al. ed.), Ilots de Langerhans et hepatocytes: vers une utilisation therapeutique, pp. 195-205. Les Editions INSERM, Paris.

    Google Scholar 

  21. Terry, C., Dhawan, A., Mitry, R. R., et al. (2006) Cryopreservation of isolated human hepatocytes for transplantation: State of the art. Cryobiology 53,149–159.

    Article  PubMed  CAS  Google Scholar 

  22. Diener, B., Utesch, D., Beer, N., et al. (1993) A method for the cryopreservation of liver parenchymal cells for studies of xenobiotics. Cryobiology 30, 116–127.

    Article  PubMed  CAS  Google Scholar 

  23. Horslen, S. P., Fox, I. J. (2004) Hepatocyte transplantation. Transplantation 77, 1481–1486.

    Article  PubMed  Google Scholar 

  24. Fox, I. J., Roy-Chowdhury, J. (2004) Hepatocyte transplantation. J Hepatol 40, 878–886.

    Article  PubMed  CAS  Google Scholar 

  25. Krishna Vanaja, D., Sivakumar, B., Jesudasan, R. A., et al. (1998) In vivo identification, survival, and functional efficacy of transplanted hepatocytes in acute liver failure mice model by FISH using Y-chromO- some probe. Cell Transpl 7, 267–273.

    Article  CAS  Google Scholar 

  26. Sutherland, D. E., Numata, M., Matas, A. J., et al. (1977) Hepatocellular transplantation in acute liver failure. Surgery 82, 124–132.

    PubMed  CAS  Google Scholar 

  27. Baumgartner, D., LaPlante-O’Neill, P. M., Sutherland, D. E., et al. (1983) Effects of intrasplenic injection of hepatocytes, hepatocyte fragments and hepatocyte culture supernatants on D-galactosamine-induced liver failure in rats. Eur Surg Res 15, 129–135.

    Article  PubMed  CAS  Google Scholar 

  28. Demetriou, A. A., Reisner, A., Sanchez, J., et al. (1988) Transplantation of microcarrier-attached hepatocytes into 90% partially hepatectomized rats. Hepatology 8, 1006–1009.

    Article  PubMed  CAS  Google Scholar 

  29. Rozga, J., Holzman, M., Moscioni, A. D., etal. (1995) Repeated intraportal hepatocyte transplantation in analbuminemic rats. Cell Transpl 4, 237–243.

    Article  CAS  Google Scholar 

  30. De Vree, J. M., Ottenhoff, R., Bosma, P. J., et al. (2000) Correction of liver disease by hepatocyte transplantation in a mouse model of progressive familial intrahepatic cholestasis. Gastroenterology 119, 1720–1730.

    Article  PubMed  CAS  Google Scholar 

  31. Laconi, E., Oren, R., Mukhopadhyay, D. K., et al. (1998) Long-term, near-total liver replacement by transplantation of isolated hepatocytes in rats treated with retrorsine. Am J Pathol 153, 319–329.

    Article  PubMed  CAS  Google Scholar 

  32. Guha, C., Parashar, B., Deb, N. J., et al. (2002) Normal hepatocytes correct serum bilirubin after repopulation of Gunn rat liver subjected to irradiation/partial resection. Hepatology 36, 354–362.

    Article  PubMed  Google Scholar 

  33. Strom, S. C., Chowdhury, J. R., Fox, I. J. (1999) Hepatocyte transplantation for the treatment of human disease. Semin Liver Dis 19, 39–48.

    Article  PubMed  CAS  Google Scholar 

  34. Grossman, M., Raper, S. E., Kozarsky, K., etal. (1994) Successful ex vivo gene therapy directed to liver in a patient with familial hypercholesterolaemia. Nat Genet 6, 335–341.

    Article  PubMed  CAS  Google Scholar 

  35. Grossman, M., Rader, D. J., Muller, D. W., et al. (1995) A pilot study of ex vivo gene therapy for homozygous familial hypercholesterolaemia. Nat Med 1, 1148–1154.

    Article  PubMed  CAS  Google Scholar 

  36. Strom, S. C., Fisher, R. A., Rubinstein, W. S., et al. (1997) Transplantation of human hepatocytes. Transpl Proc 29, 2103–2106.

    Article  CAS  Google Scholar 

  37. Stephenne, X., Najimi, M., Smets, F., et al. (2005) Cryopreserved liver cell transplantation controls ornithine transcarbamylase deficient patient while awaiting liver transplantation. Am J Transpl 5, 2058–2061.

    Article  Google Scholar 

  38. Sokal, E. M., Smets, F., Bourgois, A., et al. (2003) Hepatocyte transplantation in a 4- year-old girl with peroxisomal biogenesis disease: technique, safety, and metabolic follow-up. Transplantation 76, 735–738.

    Article  PubMed  Google Scholar 

  39. Dhawan, A., Mitry, R. R., Hughes, R. D., et al. (2004) Hepatocyte transplantation for inherited factor VII deficiency. Transplantation 78, 1812–1814.

    Article  PubMed  Google Scholar 

  40. Thompson, R., Strautnieks, S. (2001) BSEP: function and role in progressive familial intrahepatic cholestasis. Semin Liver Dis 21, 545–550.

    Article  PubMed  CAS  Google Scholar 

  41. Aronsen, K. F., Nylander, G. (1967) Use of direct protography in diagnosis of liver diseases. Radiology 88, 40–47.

    PubMed  CAS  Google Scholar 

  42. Goss, J. A., Soltes, G., Goodpastor, S. E., et al. (2003) Pancreatic islet transplantation: the radiographic approach. Transplantation 76,199–203.

    Article  PubMed  Google Scholar 

  43. Maleux, G., Gillard, P., Keymeulen, B., et al. (2005) Feasibility, safety, and efficacy of percutaneous transhepatic injection of beta-cell grafts. J Vasc Interv Radiol 16, 1693–1697.

    Article  PubMed  Google Scholar 

  44. Owen, R. J., Ryan, E. A., O’Kelly, K., et al. (2003) Percutaneous transhepatic pancreatic islet cell transplantation in type 1 diabetes mellitus: radiologic aspects. Radiology 229, 165–170.

    Article  PubMed  Google Scholar 

  45. Darwish, A. A., Sokal, E., Stephenne, X., et al. (2004) Permanent access to the portal system for cellular transplantation using an implantable port device. Liver Transpl 10, 1213–1215.

    Article  PubMed  Google Scholar 

  46. Fox, I. J., Chowdhury, J. R. (2004) Hepatocyte transplantation. Am J Transpl 4 Suppl 6, 7–13.

    Article  CAS  Google Scholar 

  47. Terry, C., Dhawan, A., Mitry, R. R., et al. (2005) Preincubation of rat and human hepatocytes with cytoprotectants prior to cryopreservation can improve viability and function upon thawing. Liver Transpl 11, 1533–1540.

    Article  PubMed  Google Scholar 

  48. Mas, V. R., Maluf, D. G., Thompson, M., et al. (2004) Engraftment measurement in human liver tissue after liver cell transplantation by short tandem repeats analysis. Cell Transpl 13, 231–236.

    Article  Google Scholar 

  49. Wang, L. J., Chen, Y. M., George, D., et al. (2002) Engraftment assessment in human and mouse liver tissue after sex-mismatched liver cell transplantation by real-time quantitative PCR for Y chromosome sequences. Liver Transpl 8, 822–828.

    Article  PubMed  Google Scholar 

  50. Rogers, W. J., Meyer, C. H., Kramer, C. M. (2006) Technology insight: in vivo cell tracking by use of MRI. Nat Clin Pract Cardiovasc Med 3, 554–562.

    Article  PubMed  CAS  Google Scholar 

  51. Soriano, H. E., Wood, R. P., Kang, D. C. (1997) Hepatocellular transplantation in children with fulminant liver failure. Hepatology 30, 239A.

    Google Scholar 

  52. Sterling, R. K., Fisher, R. A. (2001) Liver transplantation: Living donor, hepatocyte, and xenotransplantation, in (Gish, R., ed.), Current Future Treatment Therapies for Liver Disease. Clinics in Liver Disease, WB Saunders, Philadelphia.

    Google Scholar 

  53. Habibullah, C. M., Syed, I. H., Qamar, A., et al. (1994) Human fetal hepatocyte transplantation in patients with fulminant hepatic failure. Transplantation 58, 951–952.

    Article  PubMed  CAS  Google Scholar 

  54. Fisher, R. A., Strom, S. C. (2000) Human hepatocyte transplantation: Biology and therapy, in (Berry, M. N., Edwards, A. M., ed.), Hepatocyte Review, Kluwer Academic Publishers, Dordrecht, The Netherlands.

    Google Scholar 

  55. Fisher, R. A., Bu, D., Thompson, M., et al. (2000) Defining hepatocellular chimerism in a liver failure patient bridged with hepatocyte infusion. Transplantation 69, 303–307.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. Juliana Puppi
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  2. Anil Dhawan
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Editor information

Editors and Affiliations

  1. King’s College Hospital, London, UK

    Anil Dhawan

  2. School of Medicine, King’s College London, London, UK

    Robin D. Hughes

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Puppi, J., Dhawan, A. (2009). Human Hepatocyte Transplantation Overview. In: Dhawan, A., Hughes, R. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 481. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-201-4_1

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  • DOI: https://doi.org/10.1007/978-1-59745-201-4_1

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-172-1

  • Online ISBN: 978-1-59745-201-4

  • eBook Packages: Springer Protocols

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