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Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield

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Genomics Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 439))

Abstract

In many large genetic studies, the amount of available DNA can be one of the criteria for selecting samples for study. In the case of large population cohorts, selecting samples based on their DNA yield can lead to biased sample selection. In addition, many valuable clinical and research sample collections exist in which the amount of DNA is very small. Unbiased whole genome amplification (WGA) of such unique samples enables genomewide scale genetic studies that would have been impossible otherwise. Multiply primed rolling circle amplification (MPRCA) and multiple displacement amplification (MDA) methods are based on the same principle. The DNA amplification is non-PCR based and uses Φ29 DNA polymerase and random hexamer primers for unbiased whole genome amplification. MDA is used for linear DNA molecules, such as genomic DNA. This chapter reviews the various applications in which whole genome amplified DNA can be used, the types of commercial kits available, and the quality control steps necessary before using the DNA in the genetic studies.

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Author information

Authors and Affiliations

  1. National Public Health Institute, Helsinki, Finland

    Kaisa Silander & Janna Saarela

Authors
  1. Kaisa Silander
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  2. Janna Saarela
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Editor information

Editors and Affiliations

  1. Animal Health Trust, Lanwades Park, Newmarket, Suffolk, UK

    Mike Starkey

  2. Oxford Gene Technology Ltd., Oxford, UK

    Ramnath Elaswarapu

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Silander, K., Saarela, J. (2008). Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield. In: Starkey, M., Elaswarapu, R. (eds) Genomics Protocols. Methods in Molecular Biology™, vol 439. Humana Press. https://doi.org/10.1007/978-1-59745-188-8_1

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  • DOI: https://doi.org/10.1007/978-1-59745-188-8_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-871-3

  • Online ISBN: 978-1-59745-188-8

  • eBook Packages: Springer Protocols

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