Abstract
Uncoating is an essential step in the retrovirus life cycle about which little is known. Uncoating is defined as the specific dissociation of the capsid shell from the viral core in the host cell cytoplasm. In this chapter, biochemical assays for studying HIV-1 uncoating in vitro are described. These techniques have proven useful for characterizing HIV-1 mutants that exhibit defects in the uncoating step of infection.
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Acknowledgments
The author thanks the former members of his laboratory Alex Kotov and Brett Forshey who contributed to the development of the HIV-1 uncoating assay, along with the present members who assisted in compiling the methods. The NIH AIDS Research and Reference Program is gratefully acknowledged for \nobreak distributing reagents. Work in the author’s laboratory was supported by NIH grants AI040364 and AI050423.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Aiken, C. (2009). Cell-Free Assays for HIV-1 Uncoating. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_4
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DOI: https://doi.org/10.1007/978-1-59745-170-3_4
Publisher Name: Humana Press
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