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Isolation and Analysis of HIV-1 Preintegration Complexes

  • Protocol
HIV Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 485))

Abstract

A discerning feature of the retrovirus lifecycle is the covalent integration of the viral reverse transcript into a chromosome within the infected cell. Integration is required for productive infection and therefore defines the viral integrase protein of human immunodeficiency virus type 1 (HIV-1) as a bona fide target for the development of antiviral drugs in the fight against HIV/AIDS. Integrase works in the context of the viral preintegration complex (PIC), a high molecular weight nucleoprotein complex that supports the integration of its endogenous viral DNA copy made during reverse transcription into an exogenous target DNA in the test tube. PIC analyses are central to understanding the molecular mechanisms of HIV-1 integration as well as investigating the pharmacological properties of integrase inhibitors. This chapter describes techniques for isolating HIV-1 PICs from cells as well as quantifying their level of integration activity in vitro.

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Acknowledgments

I thank J.E. Daigle, K. McGee-Estrada, and N.K. Raghavendra for critically reading the manuscript. This work was supported by NIH grants AI39394, AI52014, and AI70042.

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Authors and Affiliations

  1. Department of Cancer Immunology and AIDS Dana-Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA, USA

    Alan Engelman

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  1. Alan Engelman
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Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, NY, USA

    Vinayaka R. Prasad PhD  & Ganjam V. Kalpana PhD  & 

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Engelman, A. (2008). Isolation and Analysis of HIV-1 Preintegration Complexes. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_10

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  • DOI: https://doi.org/10.1007/978-1-59745-170-3_10

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-859-1

  • Online ISBN: 978-1-59745-170-3

  • eBook Packages: Springer Protocols

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