Abstract
Bone regeneration and repair is a goal of many skeletal therapies and numerous agents positively or negatively impact these processes. New therapeutic agents and effective model systems are continually sought to identify agents and characterize their mechanisms of action are in constant demand. In addition, investigations of tumor cell—bone interaction in the skeletal metastatic microenvironment require well-defined and readily orchestrated models. This chapter describes a novel ectopic ossicle model and a vossicle modification that can be used to provide focused and rapid feedback of bone growth and bone—cellular interactions. The ossicle model is a bone marrow stromal cell (BMSC)—based model and the vossicle model is a neonatal vertebral bone transplant model. These models offer opportunities to mix and compare mesenchymal (donor derived) and hematopoietic elements (host derived). Multiple implants can be placed in one mouse to facilitate various outcome analyses, such as histomorphometry, micro-CT, gene expression studies, and cell tracking using markers such as luciferase, in response to pharma cological or genetic manipulation. Implants can also be combined with other cell types, such as cancer cells to evaluate the bone—tumor microenvironment.
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References
1. Andreassen, T. T., Ejersted, C., Oxlund, H. (1999) Intermittent parathyroid hormone (1–34) treatment enhances callus formation and mechanical strength of healing rat fractures. J Bone Miner Res 14, 960–968.
2. Holzer, G., Majeska, R. J., Lundy, M. W., et al. (1999) Parathyroid hormone enhances fracture healing. A preliminary report. Clin Orthop 366, 258–263.
3. Skriptiz, R., Aspenberg, P. (2001) Implant fixation enhanced by intermittent treatment with parathyroid hormone. J Bone Jt Surg Br 83, 437–440.
4. Kuznetsov, S. A., Friedenstein, A. J., Robey, P. G. (1997) Factors required for bone marrow stromal fibroblast colony formation in vitro. Br J Haematol 97, 437–440.
5. Krebsbach, P. H., Kuznetsov, S. A., Satomura, K., et al. (1997) Bone formation in vivo: com parison of osteogenesis by transplanted mouse and human marrow stromal fibroblasts. Transplantation 63, 1059–1069.
6. Schneider, A., Taboas, J. M., McCauley, L. K., et al. (2003) Skeletal homeostasis in tissue-engineered bone. J Orthop Res 21:859–864.
7. Pettway, G. J., Schneider, A., Koh, A. J., et al. (2005) Anabolic actions of PTH (1–34): use of a novel tissue engineering model to investigate temporal effects on bone. Bone 36, 959–970.
8. Koh, A. J., Demiralp, B., Neiva, K., et al. (2005) Cells of the osteoclast lineage as mediators of the anabolic actions of parathyroid hormone in bone. Endocrinology 146, 4584–4596.
Acknowledgments
The authors acknowledge the following individuals who have contributed to the development and/or characterization of these models: Amy Koh, Paul Krebsbach, Abraham Schneider, Ana Mattos, and Jinhui Liao. Rashesh Kapadia (Scanco) is acknowledged for the micro-CT images of the ossicle model, as is Thomas Rosol for providing the ACE-1 prostate can cer cells. This work was supported by the National Institutes of Health DK53904 and CA93900.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Pettway, G.J., McCauley, L.K. (2008). Ossicle and Vossicle Implant Model Systems. In: Westendorf, J.J. (eds) Osteoporosis. Methods In Molecular Biology™, vol 455. Humana Press. https://doi.org/10.1007/978-1-59745-104-8_7
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DOI: https://doi.org/10.1007/978-1-59745-104-8_7
Publisher Name: Humana Press
Print ISBN: 978-1-58829-828-7
Online ISBN: 978-1-59745-104-8
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