Abstract
Transcription factors play pivotal roles in the control of cell growth and differentiation in health and disease. In the post-genomic era, it has become possible to locate the regions occupied by transcription factors throughout the genome, leading to better understanding of their mechanism of action and the genes that they regulate. All methods for transcription factor location analysis utilize chromatin immunoprecipitation (ChIP). Although ChIP was initially used to test whether a protein binds to a candidate promoter in living cells, newly developed methods allow the unbiased identification of novel targets of transcription factors. This chapter describes ChIP Display, an affordable method for transcription factor location analysis. Despite being relatively low throughput compared with alternative methods such as ChIP-chip and ChIP-SAGE, ChIP Display provides even small molecular biology laboratories with the opportunity to discover novel targets of any transcription factor, for which high-quality antibodies are available.
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References
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Acknowledgments
ChIP Display was developed with NIH grant R21 AR49297 and further employed in projects supported by the NIH (RO1 DK071122 and RO1 CA109147) and the Department of Defense (PC040322). The work was conducted in a facility constructed with sup port from Research Facilities Improvement Program grant no. C06 (RR10600-01, CA62528-01, RR14514-01) from the NIH/NCRR. BF holds the J. Harold and Edna L. LaBriola Chair in Genetic Orthopaedic Research at the University of Southern California.
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Barski, A., Pregizer, S., Frenkel, B. (2008). Identification of Transcription Factor Target Genes by ChIP Display. In: Westendorf, J.J. (eds) Osteoporosis. Methods In Molecular Biology™, vol 455. Humana Press. https://doi.org/10.1007/978-1-59745-104-8_14
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DOI: https://doi.org/10.1007/978-1-59745-104-8_14
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