Abstract
RNA interference (RNAi) is a new and rapidly progressing technology for facilitating functional gene silencing. To perform highly efficient RNAi in cardiomyocytes, vascular smooth muscle cells, and vascular endothelial cells, which are known to have very low transfection efficiency, adenovirus-mediated RNAi was employed. The effects of RNAi on GAPDH transcripts were successfully reduced by nearly 90% in the primary cultured cells, indicating that adenovirus-mediated gene silencing is a promising technique for gene si lencing in cardiovascular studies. This chapter describes general guidelines for selecting RNAi target sites, construction of a shuttle vector encoding short hairpin RNAi, and generation of recombinant adenovirus.
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© 2005 Humana Press Inc.
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Kasahara, H., Aoki, H. (2005). Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes. In: Sun, Z. (eds) Molecular Cardiology. Methods in Molecular Medicine™, vol 112. Humana Press. https://doi.org/10.1007/978-1-59259-879-3_9
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DOI: https://doi.org/10.1007/978-1-59259-879-3_9
Publisher Name: Humana Press
Print ISBN: 978-1-58829-363-3
Online ISBN: 978-1-59259-879-3
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