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Strategies of Conditional Gene Expression in Myocardium

An Overview

  • Protocol
Molecular Cardiology

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 112))

Abstract

The use of specialized reporter genes to monitor real-time, tissue-specific transgene expression in animal models offers an opportunity to circumvent current limitations associated with the establishment of transgenic mouse mod els. The Cre-loxP and the tetracycline (Tet)-inducible systems are useful meth ods of conditional gene expression that allow spatial (cell-type-specific) and temporal (inducer-dependent) control. Most often, the α-myosin heavy chain (α-MHC) promoter is used in these inducible systems to restrict expression of reporter genes and transgenes to the myocardium. An overview of each induc ible system is described, along with suggested reporter genes for real-time, noninvasive imaging in the myocardium. Effective gene delivery of the induc ible gene expression system is carried out by lentiviral vectors, which offer high transduction efficiency, long-term transgene expression, and low immunogenicity. This chapter outlines the packaging of myocardium-specific in ducible expression systems into lentiviral vectors, in which a transgene and a reporter gene are transduced into cardiomyocytes. In doing so, transgene and reporter expression can be monitored/tracked with bioluminescence imaging (BLI) and positron emission tomography (PET).

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Authors and Affiliations

  1. The James Hogg iCAPTURE Center for Cardiovascular and Pulmonary Research/MRL, Department of Pathology and Laboratory Medicine, University of British Columbia-St. Paul’s Hospital, Vancouver, British Columbia, Canada

    Heather L. Heine & Hon Sing Leong

  2. Biomedical Research Centre, Department of Medical Genetics, University of British Columbia, Vancouver British Columbia, Canada

    Fabio M. V. Rossi

  3. The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research, Department of Pathology and Laboratory Medicine, St. Paul’s Hospital/Providence Health Care-University of British Columbia, and Institute of Circulatory and Respiratory Health, Canadian Institutes of Health Research, Vancouver, British Columbia, Canada

    Bruce M. McManus & Thomas J. Podor

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  1. Heather L. Heine
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  2. Hon Sing Leong
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  3. Fabio M. V. Rossi
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  4. Bruce M. McManus
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  5. Thomas J. Podor
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Editors and Affiliations

  1. Departments of Medicine and Physiology and Functional Genomics, College of Medicine, University of Florida, Gainesville, FL

    Zhongjie Sun

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L. Heine, H., Leong, H.S., Rossi, F.M.V., McManus, B.M., Podor, T.J. (2005). Strategies of Conditional Gene Expression in Myocardium. In: Sun, Z. (eds) Molecular Cardiology. Methods in Molecular Medicine™, vol 112. Humana Press. https://doi.org/10.1007/978-1-59259-879-3_8

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  • DOI: https://doi.org/10.1007/978-1-59259-879-3_8

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-363-3

  • Online ISBN: 978-1-59259-879-3

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