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Continuous Production of Butanol by Clostridium acetobutylicum Immobilized in a Fibrous Bed Bioreactor

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Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO

Part of the book series: Biotechnology for Fuels and Chemicals ((ABAB))

Abstract

We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35°C. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6 g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9 h-1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Ohio State University, 140 West 19th Avenue, Columbus, OH, 43210, USA

    Wei-Cho Huang & Shang-Tian Yang

  2. Environmental Energy Inc., PO Box 15, 1253 N. Waggoner Road, Blacklick, OH, 43004, USA

    Wei-Cho Huang & David E. Ramey

Authors
  1. Wei-Cho Huang
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  2. David E. Ramey
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  3. Shang-Tian Yang
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Corresponding author

Correspondence to Shang-Tian Yang .

Editor information

Editors and Affiliations

  1. National Renewable Energy Laboratory, USA

    Mark Finkelstein  & James D. McMillan  & 

  2. Oak Ridge National Laboratory, USA

    Brian H. Davison  & Barbara Evans  & 

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© 2004 Springer Science+Business Media New York

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Huang, WC., Ramey, D.E., Yang, ST. (2004). Continuous Production of Butanol by Clostridium acetobutylicum Immobilized in a Fibrous Bed Bioreactor. In: Finkelstein, M., McMillan, J.D., Davison, B.H., Evans, B. (eds) Proceedings of the Twenty-Fifth Symposium on Biotechnology for Fuels and Chemicals Held May 4–7, 2003, in Breckenridge, CO. Biotechnology for Fuels and Chemicals. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-837-3_72

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  • DOI: https://doi.org/10.1007/978-1-59259-837-3_72

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4684-9873-8

  • Online ISBN: 978-1-59259-837-3

  • eBook Packages: Springer Book Archive

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