Abstract
Although a large amount—perhaps still a major amount—of direct protein sequence information has come from manual sequential degradation, there is a pervasive feeling now among scientists who utilize this information that manual methods are, at best, a relic. Even among protein chemists the word “sequencer” has come to mean a machine, not a person. Compound this with the widespread opinion that protein chemistry itself has lost its status as a scientific discipline, existing as it does merely to supply probes to the molecular geneticists and occasionally to check their DNA reading frames, and one has a method in deep psychological crisis. Fortunately, manual sequencing can provide any of the structural data that requires or advantageously utilizes protein chemistry.
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Tarr, G.E. (1986). Manual Edman Sequencing System. In: Shively, J.E. (eds) Methods of Protein Microcharacterization. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-59259-436-8_6
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DOI: https://doi.org/10.1007/978-1-59259-436-8_6
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