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Sequencing and Assembling Genomes and Chromosomes of Cereal Crops

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2072))

Abstract

Next generation sequencing (NGS) and assembly are key pieces in the success of cereal crops genomes sequencing. One interesting strategy for reducing the complexity in large genomes, the case of several cereal crops, is the sequencing of individual chromosomes. This has been done with success by flow cytometric chromosome sorting followed by sequencing using available next generation (high throughput) sequencing platforms. In this chapter, methodologies for sequencing and assembly of flow sorted chromosomes and whole genomes in cereal crops, with special emphasis on the case of the International Wheat Genome Sequencing Consortium (IWGSC), are reviewed.

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Author information

Authors and Affiliations

  1. Laboratorio de Biotecnología EEA INTA Marcos Juárez, Marcos Juárez, Argentina

    Marcelo Helguera

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  1. Marcelo Helguera
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Correspondence to Marcelo Helguera .

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Editors and Affiliations

  1. Instituto de Diversidad y Ecología Animal, Consejo Nacional de Investigaciones Científicas y Técnicas (IDEA, CONICET), Universidad Nacional de Córdoba, Córdoba, Argentina

    Luis M. Vaschetto

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Helguera, M. (2020). Sequencing and Assembling Genomes and Chromosomes of Cereal Crops. In: Vaschetto, L. (eds) Cereal Genomics. Methods in Molecular Biology, vol 2072. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9865-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9865-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9864-7

  • Online ISBN: 978-1-4939-9865-4

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