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Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens

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Genotype Phenotype Coupling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2070))

Abstract

Antibody phage display is the most used in vitro technology to generate recombinant, mainly human, antibodies as tools for research, for diagnostic assays, and for therapeutics. Up to now (autumn 2018), eleven FDA/EMA-approved therapeutic antibodies were developed using phage display, including the world best-selling antibody adalimumab.

A key to generate successfully human antibodies in vitro is the choice of the most appropriate antibody selection method, for our goal. In this book chapter, we describe the antibody selection process (panning) in solution and its advantages over panning on immobilized antigens. Detailed protocols on the panning procedure and the screening of monoclonal binders are given.

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Acknowledgments

This review contains updated and revised parts of former protocols [35].

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Authors and Affiliations

  1. Institut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Braunschweig, Germany

    Esther V. Wenzel, Kristian D. R. Roth, Giulio Russo, Viola Fühner, Saskia Helmsing, André Frenzel & Michael Hust

  2. YUMAB GmbH, Science Campus Braunschweig Süd, Braunschweig, Germany

    André Frenzel & Michael Hust

Authors
  1. Esther V. Wenzel
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  2. Kristian D. R. Roth
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  3. Giulio Russo
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  4. Viola Fühner
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  5. Saskia Helmsing
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  6. André Frenzel
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  7. Michael Hust
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Corresponding author

Correspondence to Michael Hust .

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Editors and Affiliations

  1. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Stefan Zielonka

  2. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Simon Krah

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Wenzel, E.V. et al. (2020). Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens. In: Zielonka, S., Krah, S. (eds) Genotype Phenotype Coupling. Methods in Molecular Biology, vol 2070. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9853-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9853-1_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9852-4

  • Online ISBN: 978-1-4939-9853-1

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