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Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water

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Cryptosporidium

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2052))

Abstract

This chapter provides a detailed protocol to assess disinfection efficacy of chlorine against Cryptosporidium oocysts including the core chlorine disinfection assay, the in vitro cell culture infectivity assay, and microscopy analysis and data interpretation.

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Acknowledgments

The authors would like to acknowledge the expert assistance of Long Ti Xie, who helped to optimize the culture methods and parasite quantification in the immunofluorescence assays.

Disclosures: Use of trade names is for identification only and does not imply endorsement by the Public Health Service or by the U.S. Department of Health and Human Services. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention.

Author information

Authors and Affiliations

  1. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Jennifer L. Murphy & Michael J. Arrowood

Authors
  1. Jennifer L. Murphy
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  2. Michael J. Arrowood
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Corresponding author

Correspondence to Jennifer L. Murphy .

Editor information

Editors and Affiliations

  1. Atlanta Veterans Affairs Medical Center, Decatur, GA, USA

    Jan R. Mead

  2. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Michael J. Arrowood

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Murphy, J.L., Arrowood, M.J. (2020). Cell Culture Infectivity to Assess Chlorine Disinfection of Cryptosporidium Oocysts in Water. In: Mead, J., Arrowood, M. (eds) Cryptosporidium. Methods in Molecular Biology, vol 2052. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9748-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-9748-0_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9747-3

  • Online ISBN: 978-1-4939-9748-0

  • eBook Packages: Springer Protocols

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