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Quantitative Analysis of Stall of Replicating DNA Polymerase by G-Quadruplex Formation

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G-Quadruplex Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

The formation of guanine-quadruplexes (G4s) in genomic DNA and RNA inhibits replication, transcription, and translation. Although several regions of the human genome exhibit G4-forming potential, the stability and topology of the G4s formed vary depending on the molecular environment. Here, we describe a protocol to quantitatively analyze the inhibitory effects of G4s with different stabilities and topologies on replication in conditions of molecular crowding.

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Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and Japan Society for the Promotion of Science (JSPS), especially a Grant-in-Aid for Scientific Research on Innovative Areas “Chemistry for Multimolecular Crowding Biosystems” (JSPS KAKENHI Grant No. JP17H06351), JSPS foresight program, MEXT-Supported Program for the Strategic Research Foundation at Private Universities (2014–2019), Japan, The Hirao Taro Foundation of Konan Gakuen for Academic Research, The Okazaki Kazuo Foundation of Konan Gakuen for Advanced Scientific Research, and the Chubei Itoh Foundation.

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Authors and Affiliations

  1. Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan

    Shuntaro Takahashi & Naoki Sugimoto

  2. Graduate School of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan

    Naoki Sugimoto

Authors
  1. Shuntaro Takahashi
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  2. Naoki Sugimoto
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Corresponding author

Correspondence to Naoki Sugimoto .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Takahashi, S., Sugimoto, N. (2019). Quantitative Analysis of Stall of Replicating DNA Polymerase by G-Quadruplex Formation. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

  • eBook Packages: Springer Protocols

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