Abstract
Seed germination assays consist of counting the number of germinated seeds, defined as seeds in which the radicle has ruptured the endosperm and emerged from the seed coat. In Arabidopsis seed germination assays, Arabidopsis seeds are surface-sterilized, plated on agar plates containing test compounds, and incubated at specific temperatures under specific light conditions, after which the germinated seeds are counted, either with the naked eye or under a microscope. This chapter describes step-by-step protocols for Arabidopsis seed germination assays under phytochrome-dependent conditions.
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Acknowledgments
We thank the members of our laboratory for many technical tips. This work was supported in part by grants from the National Research Foundation of Korea (2018R1A3B1052617) to G.C.
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Song, K., Choi, G. (2019). Phytochrome Regulation of Seed Germination. In: Hiltbrunner, A. (eds) Phytochromes. Methods in Molecular Biology, vol 2026. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9612-4_12
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DOI: https://doi.org/10.1007/978-1-4939-9612-4_12
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