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Methods for Studying Swarming and Swimming Motility

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Proteus mirabilis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2021))

Abstract

Proteus mirabilis is well known for using its flagella to swim through liquids or swarm across solid surfaces. Both phenomena are easy to observe. Described here are two agar-based assays for studying both swimming and swarming behavior, and considerations that affect the outcome.

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References

  1. Schaffer JN, Pearson MM (2015) Proteus mirabilis and urinary tract infections. Microbiol Spectr 3(5). https://doi.org/10.1128/microbiolspec.UTI-0017-2013

  2. Macnab RM (1996) Flagella and motility. In: Neidhardt FC (ed) Escherichia coli and Salmonella: Cellular and molecular biology, vol 1, 2nd edn. ASM Press, Washington, DC, pp 123–145

    Google Scholar 

  3. Harshey RM (2003) Bacterial motility on a surface: many ways to a common goal. Annu Rev Microbiol 57:249–273. https://doi.org/10.1146/annurev.micro.57.030502.091014

    Article  CAS  PubMed  Google Scholar 

  4. Kearns DB (2010) A field guide to bacterial swarming motility. Nat Rev Microbiol 8(9):634–644. https://doi.org/10.1038/nrmicro2405

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Jones BV, Young R, Mahenthiralingam E, Stickler DJ (2004) Ultrastructure of Proteus mirabilis swarmer cell rafts and role of swarming in catheter-associated urinary tract infection. Infect Immun 72(7):3941–3950

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Armbruster CE, Prenovost K, Mobley HL, Mody L (2017) How often do clinically diagnosed catheter-associated urinary tract infections in nursing homes meet standardized criteria? J Am Geriatr Soc 65(2):395–401. https://doi.org/10.1111/jgs.14533

    Article  PubMed  Google Scholar 

  7. Warren JW, Tenney JH, Hoopes JM, Muncie HL, Anthony WC (1982) A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. J Infect Dis 146(6):719–723

    Article  CAS  PubMed  Google Scholar 

  8. Tittsler RP, Sandholzer LA (1936) The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31(6):575–580

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Armbruster CE, Hodges SA, Mobley HLT (2013) Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine. J Bacteriol 195(6):1305–1319. https://doi.org/10.1128/JB.02136-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Li X, Rasko DA, Lockatell CV, Johnson DE, Mobley HLT (2001) Repression of bacterial motility by a novel fimbrial gene product. EMBO J 20(17):4854–4862

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Pearson MM, Mobley HLT (2008) Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis. Mol Microbiol 69(2):548–558

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Coetzee JN, Sacks TG (1960) Morphological variants of Proteus hauseri. J Gen Microbiol 23:209–216. https://doi.org/10.1099/00221287-23-2-209

    Article  CAS  PubMed  Google Scholar 

  13. Pearson MM, Rasko DA, Smith SN, Mobley HLT (2010) Transcriptome of swarming Proteus mirabilis. Infect Immun 78(6):2834–2845. https://doi.org/10.1128/IAI.01222-09

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Allison C, Lai HC, Hughes C (1992) Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis. Mol Microbiol 6(12):1583–1591

    Article  CAS  PubMed  Google Scholar 

  15. Walker KE, Moghaddame-Jafari S, Lockatell CV, Johnson D, Belas R (1999) ZapA, the IgA-degrading metalloprotease of Proteus mirabilis, is a virulence factor expressed specifically in swarmer cells. Mol Microbiol 32(4):825–836

    Article  CAS  PubMed  Google Scholar 

  16. Alteri CJ, Himpsl SD, Engstrom MD, Mobley HLT (2012) Anaerobic respiration using a complete oxidative TCA cycle drives multicellular swarming in Proteus mirabilis. mBio 3(6). doi:https://doi.org/10.1128/mBio.00365-12

  17. Allison C, Lai HC, Gygi D, Hughes C (1993) Cell differentiation of Proteus mirabilis is initiated by glutamine, a specific chemoattractant for swarming cells. Mol Microbiol 8(1):53–60

    Article  CAS  PubMed  Google Scholar 

  18. Rauprich O, Matsushita M, Weijer CJ, Siegert F, Esipov SE, Shapiro JA (1996) Periodic phenomena in Proteus mirabilis swarm colony development. J Bacteriol 178(22):6525–6538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Kvittingen J (1949) Studies of the life-cycle of Proteus Hauser. Acta Pathol Mic Sc 26(1):24–50

    Article  CAS  Google Scholar 

  20. Pearson MM, Sebaihia M, Churcher C, Quail MA, Seshasayee AS, Luscombe NM, Abdellah Z, Arrosmith C, Atkin B, Chillingworth T, Hauser H, Jagels K, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Walker D, Whithead S, Thomson NR, Rather PN, Parkhill J, Mobley HLT (2008) Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility. J Bacteriol 190(11):4027–4037

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Daum B, Gold V (2018) Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint. Biol Chem 399(7):799–808. https://doi.org/10.1515/hsz-2018-0157

    Article  CAS  PubMed  Google Scholar 

  22. Hazelbauer GL, Mesibov RE, Adler J (1969) Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc Natl Acad Sci U S A 64(4):1300–1307

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Belas R, Schneider R, Melch M (1998) Characterization of Proteus mirabilis precocious swarming mutants: identification of rsbA, encoding a regulator of swarming behavior. J Bacteriol 180(23):6126–6139

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Gygi D, Rahman MM, Lai HC, Carlson R, Guard-Petter J, Hughes C (1995) A cell-surface polysaccharide that facilitates rapid population migration by differentiated swarm cells of Proteus mirabilis. Mol Microbiol 17(6):1167–1175

    Article  CAS  PubMed  Google Scholar 

  25. Matsuyama T, Takagi Y, Nakagawa Y, Itoh H, Wakita J, Matsushita M (2000) Dynamic aspects of the structured cell population in a swarming colony of Proteus mirabilis. J Bacteriol 182(2):385–393

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

Thank you to Stephanie Himpsl and Chelsie Armbruster for adding suggestions to these methods.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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  1. Melanie M. Pearson
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Correspondence to Melanie M. Pearson .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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Pearson, M.M. (2019). Methods for Studying Swarming and Swimming Motility. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9601-8_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9600-1

  • Online ISBN: 978-1-4939-9601-8

  • eBook Packages: Springer Protocols

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