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Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes

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Lipid-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2003))

Abstract

Surface plasmon resonance (SPR) is an established method for studying molecular interactions in real time. It allows obtaining qualitative and quantitative data on interactions of proteins with lipids or lipid membranes. In most of the approaches a lipid membrane or a membrane-mimetic surface is prepared on the surface of Biacore (GE Healthcare) sensor chips HPA or L1, and the studied protein is then injected across the surface. Here we provide an overview of SPR in protein–lipid and protein–membrane interactions, different approaches described in the literature and a general protocol for conducting an SPR experiment including lipid membranes, together with some experimental considerations.

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Acknowledgments

We would like to thank the Slovenian Research Agency for the continuing support of the Infrastructural Centre for Molecular Interaction Analysis at the Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia (program grant number I0-0022) and for the support of the program grant “Molecular interactions” (grant number P1-0391).

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Authors and Affiliations

  1. Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, Ljubljana, Slovenia

    Aleksandra Šakanovič, Vesna Hodnik & Gregor Anderluh

  2. Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia

    Vesna Hodnik

Authors
  1. Aleksandra Šakanovič
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  2. Vesna Hodnik
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  3. Gregor Anderluh
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Corresponding author

Correspondence to Gregor Anderluh .

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Editors and Affiliations

  1. Department of Biophysics, Institute of Biology, FB10 and Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Kassel, Germany

    Jörg H. Kleinschmidt

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Šakanovič, A., Hodnik, V., Anderluh, G. (2019). Surface Plasmon Resonance for Measuring Interactions of Proteins with Lipids and Lipid Membranes. In: Kleinschmidt, J. (eds) Lipid-Protein Interactions. Methods in Molecular Biology, vol 2003. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9512-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9512-7_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9511-0

  • Online ISBN: 978-1-4939-9512-7

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