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Three-Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software

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The ESCRT Complexes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1998))

Abstract

Visualization of subcellular localization of ESCRT proteins and their interactions with different cellular compartments are critical to understand their function. This approach requires the generation of an important amount of 3D fluorescence microscopy data that is not always easy to visualize and apprehend.

We describe a step-by-step protocol for 3D surface rendering of confocal microscopy acquisitions using the free software UCSF-Chimera, generating snapshots and animations to facilitate analysis and presentation of subcellular localization data.

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Acknowledgments

All figures were captured with the UCSF Chimera package. Chimera is developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco (supported by NIGMS P41-GM103311).

The present work has benefited from the light microscopy facility of Imagerie-Gif, member of IBiSA and supported by “France-BioImaging” (ANR-10-INBS-04-01).

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Authors and Affiliations

  1. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Romain Le Bars, Michele W. Bianchi & Christophe Lefebvre

  2. Faculté des Sciences et Technologie, Université Paris-Est Créteil, Créteil, France

    Michele W. Bianchi

Authors
  1. Romain Le Bars
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  2. Michele W. Bianchi
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  3. Christophe Lefebvre
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Corresponding author

Correspondence to Romain Le Bars .

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Editors and Affiliations

  1. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Emmanuel Culetto

  2. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ.Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France

    Renaud Legouis

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Le Bars, R., Bianchi, M.W., Lefebvre, C. (2019). Three-Dimensional Surface Rendering of ESCRT Proteins Microscopy Data Using UCSF Chimera Software . In: Culetto, E., Legouis, R. (eds) The ESCRT Complexes. Methods in Molecular Biology, vol 1998. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9492-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9492-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9491-5

  • Online ISBN: 978-1-4939-9492-2

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