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CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification

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Single Cell Methods

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1979))

Abstract

Single-cell RNA sequencing has revolutionized the way we look at cell populations. Of the methods available, CEL-Seq was the first to use linear RNA amplification. With early barcoding and 3′ sequencing, it is sensitive, cost-effective and easy to perform. Here we describe a protocol for performing CEL-Seq2 on sorted cells, which can be performed without any special equipment.

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Acknowledgments

We thank the Technion Genome center for technical assistance.

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Authors and Affiliations

  1. Institute for Computational Medicine, NYU School of Medicine, New York, NY, USA

    Itai Yanai

  2. Lokey Interdisciplinary Center for Life Sciences and Engineering, Technion–Israel Institute of Technology, Haifa, Israel

    Tamar Hashimshony

Authors
  1. Itai Yanai
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  2. Tamar Hashimshony
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Corresponding author

Correspondence to Tamar Hashimshony .

Editor information

Editors and Affiliations

  1. Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy

    Valentina Proserpio

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Yanai, I., Hashimshony, T. (2019). CEL-Seq2—Single-Cell RNA Sequencing by Multiplexed Linear Amplification. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9240-9_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9239-3

  • Online ISBN: 978-1-4939-9240-9

  • eBook Packages: Springer Protocols

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