Abstract
We provide a DNA extraction protocol optimized for the recovery of highly fragmented molecules preserved within bones and teeth. In this method, the hard tissue matrix is degraded using an EDTA/Proteinase K lysis buffer, and the DNA is purified using spin columns with silica membranes. This method efficiently recovers molecules as short as 35 base-pairs long.
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References
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Dabney, J., Meyer, M. (2019). Extraction of Highly Degraded DNA from Ancient Bones and Teeth. In: Shapiro, B., Barlow, A., Heintzman, P., Hofreiter, M., Paijmans, J., Soares, A. (eds) Ancient DNA. Methods in Molecular Biology, vol 1963. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9176-1_4
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DOI: https://doi.org/10.1007/978-1-4939-9176-1_4
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9175-4
Online ISBN: 978-1-4939-9176-1
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