Abstract
Adeno-associated virus (AAV) vectors are an efficient method of gene delivery to various tissues including the lung. Mouse models are often used as a preliminary preclinical model in order to advance AAV lung gene therapy vectors. In this chapter we describe an AAV purification protocol using heparin affinity chromatography as well as an intranasal and intratracheal method of delivering AAV vectors to the lungs of mice.
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Acknowledgments
This work was supported by the Lung Association – Ontario and Cystic Fibrosis Canada.
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van Lieshout, L.P., Domm, J.M., Wootton, S.K. (2019). AAV-Mediated Gene Delivery to the Lung. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_21
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DOI: https://doi.org/10.1007/978-1-4939-9139-6_21
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Online ISBN: 978-1-4939-9139-6
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