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AAV-Mediated Gene Delivery to Taste Cells of the Tongue

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Adeno-Associated Virus Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1950))

Abstract

Taste sensation is initiated in sensory cells within the taste buds (taste cells), in which the cooperation of many signaling molecules leads to the coding and transmission of information on the quality and intensity of taste to the afferent gustatory nerves. Here, we describe our method for inducing foreign gene expression in taste cells of fungiform papillae in a living mouse using a recombinant adeno-associated virus (AAV) vector, enabling us to study and control the function of a gene product in vivo. Among the serotypes tested to date, only AAV-DJ, a synthetic serotype, can transduce taste cells in vivo. We also describe how to validate intragemmal foreign gene expression in fungiform taste buds using an immunohistochemical approach.

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Acknowledgments

This work was supported by Grants-in-Aid from Japan Society of the Promotion of Science [26713008 and 16K15181 to A.T.; 15K18974 to M.K.]; Salt Science [1429 and 1542 to A.T.; 1634 to M.K.].

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Authors and Affiliations

  1. Department of Molecular Cell Physiology, Kyoto Prefectural University of Medicine, Kyoto, Japan

    Akiyuki Taruno

  2. Department of Physiology, Aichi Medical University, Nagakute, Japan

    Makiko Kashio

Authors
  1. Akiyuki Taruno
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  2. Makiko Kashio
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Corresponding author

Correspondence to Akiyuki Taruno .

Editor information

Editors and Affiliations

  1. Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA

    Michael J. Castle

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Taruno, A., Kashio, M. (2019). AAV-Mediated Gene Delivery to Taste Cells of the Tongue. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9139-6_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9138-9

  • Online ISBN: 978-1-4939-9139-6

  • eBook Packages: Springer Protocols

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