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AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection

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Adeno-Associated Virus Vectors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1950))

Abstract

Gene therapy targeting the spinal cord is an important tool for analyzing mechanisms of nervous system diseases and the development of gene therapies. Analogous to a lumbar puncture in humans, the rodent spinal cord can be accessed through an efficient, noninvasive injection. Here we describe a method for AAV-mediated gene transfer to cells of the spinal cord by intrathecal injection of small quantities of AAV vector.

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Acknowledgments

This work was supported by DoD grant W81XWH-15-1-0494 to C.P. and C.F., F32 NS 100438 to A.G.J.S., R01 DE021996 to L.V.

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Author notes

  1. Cristina D. Peterson and Alexander G. J. Skorput contributed equally to this work.

Authors and Affiliations

  1. Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA

    Cristina D. Peterson, Alexander G. J. Skorput, Kelley F. Kitto, George L. Wilcox, Lucy Vulchanova & Carolyn A. Fairbanks

  2. Department of Pharmacology, University of Minnesota, Minneapolis, MN, USA

    George L. Wilcox & Carolyn A. Fairbanks

  3. Department of Dermatology, University of Minnesota, Minneapolis, MN, USA

    George L. Wilcox

  4. Department of Pharmaceutics, University of Minnesota, Minneapolis, MN, USA

    Carolyn A. Fairbanks

Authors
  1. Cristina D. Peterson
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  2. Alexander G. J. Skorput
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  3. Kelley F. Kitto
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  4. George L. Wilcox
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  5. Lucy Vulchanova
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  6. Carolyn A. Fairbanks
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Corresponding author

Correspondence to Carolyn A. Fairbanks .

Editor information

Editors and Affiliations

  1. Department of Neurosciences, University of California, San Diego, La Jolla, CA, USA

    Michael J. Castle

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Peterson, C.D., Skorput, A.G.J., Kitto, K.F., Wilcox, G.L., Vulchanova, L., Fairbanks, C.A. (2019). AAV-Mediated Gene Delivery to the Spinal Cord by Intrathecal Injection. In: Castle, M. (eds) Adeno-Associated Virus Vectors. Methods in Molecular Biology, vol 1950. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9139-6_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9139-6_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9138-9

  • Online ISBN: 978-1-4939-9139-6

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