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Miniaturized Checkerboard Assays to Measure Antibiotic Interactions

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Bioinformatics and Drug Discovery

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1939))

Abstract

Drugs may have synergistic or antagonistic interactions when combined. Checkerboard assays, where two drugs are combined in many doses, allow sensitive measurement of drug interactions. Here, we describe a protocol to measure the pairwise interactions among three antibiotics, in duplicate, in 5 days, using only two 96-well microplates and standard laboratory equipment.

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Author information

Authors and Affiliations

  1. Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla, Istanbul, Turkey

    Melike Cokol-Cakmak

  2. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA

    Murat Cokol

  3. Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA

    Murat Cokol

Authors
  1. Melike Cokol-Cakmak
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  2. Murat Cokol
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Corresponding author

Correspondence to Murat Cokol .

Editor information

Editors and Affiliations

  1. Department of Pathology, University of New Mexico, Albuquerque, NM, USA

    Richard S. Larson

  2. Department of Internal Medicine, University of New Mexico, Albuquerque, NM, USA

    Tudor I. Oprea

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Cokol-Cakmak, M., Cokol, M. (2019). Miniaturized Checkerboard Assays to Measure Antibiotic Interactions. In: Larson, R., Oprea, T. (eds) Bioinformatics and Drug Discovery. Methods in Molecular Biology, vol 1939. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9089-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9089-4_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9088-7

  • Online ISBN: 978-1-4939-9089-4

  • eBook Packages: Springer Protocols

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