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Description of Ovariectomy Protocol in Mice

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Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Estrogen and ovarian function decline are relevant characteristics of menopause period. Numerous physiological, metabolic and immunological alterations in the female body occur in the menopause period and some of these changes remain uncertain. The animal model that mimics menopause phase is an important approach to better comprehend the biological process involved in this period of women life. Ovariectomy is a procedure where ovaries are surgically excised and have been a valuable tool for understanding estrogen deficiency through animal experiments. Despite the diversity of ovariectomy protocols, the aim of this chapter is to provide a comprehensive guideline in performing ovariectomy in mice. Furthermore, isoflurane anesthesia system, postoperative care and surgery success evaluation will be described. We highlight that all procedures must be carried out by a qualified and trained professional, respecting ethical and safety principles.

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Acknowledgments

This study was funded by São Paulo Research Foundation (FAPESP), project number 2012/50410-8 to CMF; 2012/50336-2 and 2016/50484-2 to FAO. CNPq (The National Council for Scientific and Technological Development), project number 486037/2012-6 to CMF. Also, the authors would like to acknowledge for the fellowships provided by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) -Finance Code 001 to VRS and EM; São Paulo Research Foundation (FAPESP), project number 2016/13496-2 to MCC.

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Author notes

  1. Vanessa R. Souza and Eduardo Mendes contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Institute of Environmental, Chemistry and Pharmaceutical Sciences, Universidade Federal de São Paulo, Diadema, SP, Brazil

    Vanessa R. Souza, Eduardo Mendes, Mateus Casaro & Caroline M. Ferreira

  2. Department of Pathology, Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil

    Ana Tada F. B. Antiorio

  3. Cellular and Molecular Neurobiology Laboratory (LaNeC), Center for Mathematics, Computing and Cognition (CMCC), Federal University of ABC–UFABC, São Bernardo do Campo, SP, Brazil

    Fernando A. Oliveira

  4. Department of Neuroscience, Carleton University, Ottawa, ON, Canada

    Fernando A. Oliveira

Authors
  1. Vanessa R. Souza
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  2. Eduardo Mendes
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  3. Mateus Casaro
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  4. Ana Tada F. B. Antiorio
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  5. Fernando A. Oliveira
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  6. Caroline M. Ferreira
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Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Souza, V.R., Mendes, E., Casaro, M., Antiorio, A.T.F.B., Oliveira, F.A., Ferreira, C.M. (2019). Description of Ovariectomy Protocol in Mice. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_29

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_29

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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