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Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence

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Autoantibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1901))

Abstract

The eventual presence of anti-neutrophil cytoplasmic antibodies (ANCA) can initially be screened with indirect immunofluorescence (IIF). The majority of laboratories that facilitate ANCA testing use commercial kits. Although in-house assays are not encouraged in routine clinical laboratories, knowledge on the methodological aspects of the assay remains of importance. These aspects include choice of substrate, choice of fixative, staining procedure, and interpretation procedure. In this paper details on the methodology are provided and discussed in the context of the clinical application.

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Abbreviations

AAV:

ANCA-associated vasculitis

ANA:

Anti-nuclear antibodies

ANCA:

Anti-neutrophil cytoplasmic antibodies

BPI:

Bactericidal permeability increasing protein

CF:

Cystic fibrosis

EGPA:

Eosinophilic granulomatosis with polyangiitis

FITC:

Fluorescein isothiocyanate

GPA:

Granulomatosis with polyangiitis

IBD:

Inflammatory bowel disease

IIF:

Indirect immunofluorescence

MPA:

Microscopic polyangiitis

MPO:

Myeloperoxidase

PR3:

Proteinase 3

PSC:

Primary sclerosing cholangitis

RA:

Rheumatoid arthritis

SLE:

Systemic lupus erythematosus

SVV:

Small vessel vasculitis

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Acknowledgments

The authors would like to acknowledge Kai Fechner (Euroimmun AG, Germany) for providing the image of formalin-fixed granulocytes.

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Authors and Affiliations

  1. Central Diagnostic Laboratory, Maastricht University Medical Centre, Maastricht, The Netherlands

    J. J. B. C. van Beers, J. Vanderlocht & J. Damoiseaux

  2. Department of Laboratory Medicine, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands

    C. Roozendaal

Authors
  1. J. J. B. C. van Beers
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  2. J. Vanderlocht
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  3. C. Roozendaal
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  4. J. Damoiseaux
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Corresponding author

Correspondence to J. Damoiseaux .

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Editors and Affiliations

  1. Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark

    Gunnar Houen

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van Beers, J.J.B.C., Vanderlocht, J., Roozendaal, C., Damoiseaux, J. (2019). Detection of Anti-neutrophil Cytoplasmic Antibodies (ANCA) by Indirect Immunofluorescence. In: Houen, G. (eds) Autoantibodies. Methods in Molecular Biology, vol 1901. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8949-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8949-2_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8948-5

  • Online ISBN: 978-1-4939-8949-2

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