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Immunohistochemistry to Study YAP in Human Tissue Samples

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

Immunohistochemistry (IHC) analysis of YAP in human tissue samples represents an important means to analyze overall expression levels and subcellular localization of YAP in specimen of interest. As transcriptional coactivator, alterations of YAP levels in the cellular nucleus allow important predictions for YAP activity and transcriptional state of target genes. In the following report, IHC procedures optimized for the detection of YAP in tissue slides of FFPE material are provided. Of note, de-paraffinization and heat-induced antigen retrieval are strictly necessary for successful YAP IHC staining. Further, immunostaining using a labelled polymer-HRP system combined with diaminobenzidine (DAB), as signal-amplifying chromogen, allows strong staining results with minimal unspecific background signal.

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Acknowledgments

The authors acknowledge funding from NIH/NCI R01CA211052 and NIH/NCI R01CA204302 (to T.G.B.) as well as from the German Cancer Aid (Mildred Scheel postdoctoral fellowship, to F.H.).

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Author notes

  1. Franziska Haderk and Victor Olivas contributed equally to this work.

Authors and Affiliations

  1. Department of Medicine, University of California, San Francisco, San Francisco, CA, USA

    Franziska Haderk, Victor Olivas & Trever G. Bivona

  2. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA, USA

    Franziska Haderk, Victor Olivas & Trever G. Bivona

  3. Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA, USA

    Franziska Haderk, Victor Olivas & Trever G. Bivona

Authors
  1. Franziska Haderk
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  2. Victor Olivas
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  3. Trever G. Bivona
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Corresponding author

Correspondence to Trever G. Bivona .

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Editors and Affiliations

  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Haderk, F., Olivas, V., Bivona, T.G. (2019). Immunohistochemistry to Study YAP in Human Tissue Samples. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8909-6

  • Online ISBN: 978-1-4939-8910-2

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