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Inducible Protein Production in 293 Cells Using the piggyBac Transposon System

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Recombinant Protein Expression in Mammalian Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1850))

Abstract

Recombinant proteins are widely used to study various pathophysiological processes. Nevertheless, the establishment of the desired protein-producing stable mammalian cell lines using traditional methods is hampered by multiple laborious steps. In this chapter, we describe a simple and robust system that allows for the derivation of stable transgenic cell lines in 293 cells, yielding high protein expression levels, in a short time period. This methodology is based on the piggyBac transposon system and, notably, it allows for inducible production of the protein of interest. Moreover, it can easily be used in conventional laboratory cell culture settings and does not require any specialized devices. Herein, we outline all the steps of this procedure in detail and point out specific considerations.

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Author information

Authors and Affiliations

  1. Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    Iacovos P. Michael

  2. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada

    Andras Nagy

  3. Department of Obstetrics and Gynaecology, Institute of Medical Science, University of Toronto, Toronto, ON, Canada

    Andras Nagy

Authors
  1. Iacovos P. Michael
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  2. Andras Nagy
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Corresponding author

Correspondence to Iacovos P. Michael .

Editor information

Editors and Affiliations

  1. Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland

    David L. Hacker

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Michael, I.P., Nagy, A. (2018). Inducible Protein Production in 293 Cells Using the piggyBac Transposon System. In: Hacker, D. (eds) Recombinant Protein Expression in Mammalian Cells. Methods in Molecular Biology, vol 1850. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8730-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8730-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8729-0

  • Online ISBN: 978-1-4939-8730-6

  • eBook Packages: Springer Protocols

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