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Identification and Quantification of DNA Viral Populations in Human Urine Using Next-Generation Sequencing Approaches

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The Human Virome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1838))

Abstract

The human urinary virome is comprised by diverse communities of both eukaryotic viruses and bacteriophages. Identification and quantification of human urinary viruses was limited to PCR-based and culture methods. Detection of viruses in human urine has long been associated with disease, but advances in next-generation sequencing have shown that it harbors robust viral communities. Human urinary viral communities are mainly comprised by bacteriophages and Human Papillomaviruses (HPVs) that remain to be characterized in association with health and diverse urinary disease states. In the present chapter, the methods to identify and quantify DNA viral populations in human urine using next-generation sequencing are described.

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Correspondence to Tasha M. Santiago-Rodriguez .

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Santiago-Rodriguez, T.M. (2018). Identification and Quantification of DNA Viral Populations in Human Urine Using Next-Generation Sequencing Approaches. In: Moya, A., Pérez Brocal, V. (eds) The Human Virome. Methods in Molecular Biology, vol 1838. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8682-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8682-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8681-1

  • Online ISBN: 978-1-4939-8682-8

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