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3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virus-Host Interactions

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Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1836))

Abstract

Viruses use different strategies to interact with their host and perform a successful viral infection that results in the formation of new infectious viral particles and their propagation to new hosts. Understanding how viruses interact with their hosts requires the use of high-resolution techniques for the direct visualization of these interactions. Here electron microscopy (EM) methods are described that allow the 3D ultrastructural analysis of virus-infected cells. These methods can be implemented with light microscopy (LM) to certainly allocate virus-infected cells or cells displaying a specific/interesting phenotype caused by the interaction of viral proteins with the cellular machinery. Some sample preparation procedures where LM is integrated, known as correlative light electron microscopy (CLEM), are also explained in this chapter. All of these methods are applicable to any kind of cultured cells, including influenza virus-infected cells.

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Acknowledgments

Thank you to the excellent technical support provided by Uta Haselmann and the staff members of the Electron Microscopy Core Facility at the European Molecular Biology Laboratory (EMBL, Heidelberg) and at the University of Heidelberg. Special thanks to Nicole Schieber and Rachel Mellwig (EMBL, Heidelberg) for their critical reading of this manuscript and their helpful suggestions. I also would like to express my gratitude to Prof. Dr. Ralf Bartenschlager, chief of the Molecular Virology Department at the University of Heidelberg for his great support, as well as to all the members of the department. Also to Dr. Yannick Schwab and his team at EMBL, specially to Dr. Anna Steyer.

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Authors and Affiliations

  1. Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany

    Inés Romero-Brey

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  1. Inés Romero-Brey
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Correspondence to Inés Romero-Brey .

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Editors and Affiliations

  1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Yohei Yamauchi

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Romero-Brey, I. (2018). 3D Electron Microscopy (EM) and Correlative Light Electron Microscopy (CLEM) Methods to Study Virus-Host Interactions. In: Yamauchi, Y. (eds) Influenza Virus. Methods in Molecular Biology, vol 1836. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8678-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8678-1_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8677-4

  • Online ISBN: 978-1-4939-8678-1

  • eBook Packages: Springer Protocols

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