Abstract
Ovine amniotic epithelial cells (oAEC) are a subset of placental stem cells with great regenerative and immunomodulatory properties. Indeed, oAEC are object of intense study for regenerative medicine thanks to the several advantages in developing pre-clinical studies on a high value translational animal model, such as sheep. For this reason, a critical standardization of cultural practices is fundamental to preserve in vitro oAEC native phenotype, thus improving theirĀ in vivo therapeutic potential and clinical outcomes. Here is described an improved oAEC cultural protocol able to preserve the native epithelial phenotype also after the in vitro amplification.
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Acknowledgement
The authors acknowledge Maura Turriani for excellent technical advices in cell culture paragraph and Davide Perruzza for technical support in micrographs acquisition. This work was supported by PRIN 2015 (PRIN C42F15000180001) financed by the Italian Ministry of Education, University and Research (M.I.U.R.).
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Canciello, A., Greco, L., Russo, V., Barboni, B. (2018). Amniotic Epithelial Cell Culture. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 1817. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8600-2_7
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DOI: https://doi.org/10.1007/978-1-4939-8600-2_7
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