Abstract
The Langendorff Perfused Heart Model is an experimental procedure developed at the end of the nineteenth century by Oskar Langendorff. In this procedure, an excised heart has a cannula inserted into its aorta so that the heart can be retrogradely perfused via the coronary artery. The procedure has been improved in recent times, and these improvements are used to evaluate the direct effect of medication on the heart as well as the effect of ischemia–reperfusion injury on heart function. In this chapter, we describe protocols for evaluating heart function in Langendorff perfused rat heart.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Langendorff O (1895) Untersuchugen am überlebenden Säugethierherzen. Pflügers Arch 61:291–307
Frommeyer G, Milberg P, Witte P et al (2011) A new mechanism preventing proarrhythmia in chronic heart failure: rapid phase-III repolarization explains the low proarrhythmic potential of amiodarone in contrast to sotalol in a model of pacing-induced heart failure. Eur J Heart Fail 13:1060–1069
Kazusa K, Nakamura Y, Watanabe Y et al (2014) Effects of pH on nifekalant-induced electrophysiological change assessed in the Langendorff heart model of Guinea pigs. J Pharmacol Sci 124:153–159
Murry CE, Jennings RB, Reimer KA (1986) Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 74:1124–1136
Watanabe M, Okada T (2003) Lysophosphatidylcholine-induced myocardial damage is inhibited by pretreatment of poloxamer 188 in isolated rat heart. Mol Cell Biochem 248:209–215
Watanabe M, Shinohara A, Matsukawa T et al (2011) Chronic magnesium deficiency decreases tolerance to hypoxia/reoxygenation injury in mouse heart. Life Sci 88:658–663
Frank JS, Rich TL, Beydler S, Kreman M (1982) Calcium depletion in rabbit myocardium. Ultrastructure of the sarcolemma and correlation with the calcium paradox. Circ Res 51:117–130
Kakigi R, Watanabe M, Iesaki T et al (2016) Acute exercise preserves cardiac function against ischemia-reperfusion injury. J Physiol Sci 66:S185
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Watanabe, M., Okada, T. (2018). Langendorff Perfusion Method as an Ex Vivo Model to Evaluate Heart Function in Rats. In: Ishikawa, K. (eds) Experimental Models of Cardiovascular Diseases. Methods in Molecular Biology, vol 1816. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8597-5_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8597-5_8
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8596-8
Online ISBN: 978-1-4939-8597-5
eBook Packages: Springer Protocols