Abstract
High-speed atomic force microscopy allows for directly observing biological molecules in dynamic action at submolecular and sub-100 ms spatiotemporal resolution, without disturbing their function. This microscopy has recently been applied to various proteins with great success. Here, we describe methods to image myosin V molecules walking on actin filaments with high-speed atomic force microscopy.
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References
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Acknowledgments
This work was supported by a Grant-in-Aid for Basic Research (S) to TA form JSPS, PRESTO, JST to NK and CREST, JST to TA. We thank T. Uchihashi and D. Yamamoto for technical assistance.
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Kodera, N., Ando, T. (2018). Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy. In: Lavelle, C. (eds) Molecular Motors. Methods in Molecular Biology, vol 1805. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8556-2_6
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DOI: https://doi.org/10.1007/978-1-4939-8556-2_6
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