Skip to main content
SpringerLink
Log in

General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol

  • Protocol
  • First Online:
Teratogenicity Testing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1797))

Abstract

Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. In whole-mount IHC, the distribution of an antigen can be mapped rapidly and reliably in the embryos. Immunofluorescence microscopy is one of the numerous IHC methods that may be used to assess both the localization and endogenous levels of proteins of interest. In this chapter, a general protocol for whole-mount immunofluorescent labeling of zebrafish embryos and larvae is described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Chen X, Cho DB, Yang PC (2010) Double staining immunohistochemistry. North. Am J Med Sci 2:241–245

    Google Scholar 

  2. Inoue D, Wittbrodt J (2011) One for all – a highly efficient and versatile method for fluorescent immunostaining in fish embryos. PLoS One 6:e19713

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Fernandez J, Fuentes R (2013) Fixation/permeabilization: new alternative procedure for immunofluorescence and mRNA in situ hybridization of vertebrate and invertebrate embryos. Dev Dyn 242:503–517

    Article  CAS  PubMed  Google Scholar 

  4. Luzio A, Matos M, Santos D et al (2016) Disruption of apoptosis pathways involved in zebrafish gonad differentiation by 17α-ethinylestradiol and fadrozole exposures. Aquat Toxicol 177:269–284

    Article  CAS  PubMed  Google Scholar 

  5. Macdonald R (1999) Zebrafish immunohistochemistry. In: Guille M (ed) Molecular methods in developmental biology: Xenopus and Zebrafish, Methods in molecular biology, vol 127. Humana Press Inc., Totowa, NJ

    Chapter  Google Scholar 

  6. Thisse C, Thisse B (2008) High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc 3:59–69

    Article  CAS  PubMed  Google Scholar 

  7. Kogata N, Beatrice A, Howard BA (2013) A whole-mount immunofluorescence protocol for three-dimensional imaging of the embryonic mammary primordium. J Mammary Gland Biol Neoplasia 18:227–231

    Article  PubMed  Google Scholar 

Download references

Acknowledgment

This work is supported by European Investment Funds by FEDER/COMPETE/POCI-Operacional Competitiveness and Internacionalization Programme, under Project POCI-01-0145-FEDER-006958 and National Funds by FCT—Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. It was also supported by the FCT-PhD grant (PD/BD/127992/2016) attributed to Dércia Santos.

Author information

Authors and Affiliations

  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Universidade de Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    Dércia Santos, Sandra M. Monteiro & Ana Luzio

Authors
  1. Dércia Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sandra M. Monteiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ana Luzio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ana Luzio .

Editor information

Editors and Affiliations

  1. Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal

    Luís Félix

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Santos, D., Monteiro, S.M., Luzio, A. (2018). General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7883-0_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7882-3

  • Online ISBN: 978-1-4939-7883-0

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation