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Transcriptome Sequencing: RNA-Seq

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Computational Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1754))

Abstract

RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of different platforms are not exactly the same; each platform has its own kit to meet the special requirements of the instrument design.

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Zhang, H., He, L., Cai, L. (2018). Transcriptome Sequencing: RNA-Seq. In: Huang, T. (eds) Computational Systems Biology. Methods in Molecular Biology, vol 1754. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7717-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7717-8_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7716-1

  • Online ISBN: 978-1-4939-7717-8

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