Abstract
Here we describe a protocol to utilize human benign leiomyoma tissue in in vitro 3D model that enables an assessment of cell invasion. The chapter also describes detailed instructions for image analysis to quantify the results. Leiomyoma is a benign tumor of the uterus which mimics authentic components of the tumor microenvironment including fibroblasts, vessels, collagen fibers, and extracellular protein composition. The leiomyoma invasion model represents a superior 3D model for cell invasion studies compared to the other non-human organotypic models.
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Acknowledgments
This work was supported by Sigrid Juselius Foundation, the Finnish Cancer Foundation, the University of Helsinki Central Hospital, and the Health Science Council of the Academy of Finland (Centre of Excellence 2012–2017 Grant 251314). We thank Dr. Sini Nurmenniemi and Dr. Tatu Pirkola for developing the myoma model and the protocol of invasion quantification, respectively. We thank Mr. Donald Smart for the editorial assistance.
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Åström, P., Heljasvaara, R., Nyberg, P., Al-Samadi, A., Salo, T. (2018). Human Tumor Tissue-Based 3D In Vitro Invasion Assays. In: Cal, S., Obaya, A. (eds) Proteases and Cancer. Methods in Molecular Biology, vol 1731. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7595-2_19
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DOI: https://doi.org/10.1007/978-1-4939-7595-2_19
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7595-2
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