Abstract
Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.
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References
Basil MC, Levy BD (2016) Specialized pro-resolving mediators: endogenous regulators of infection and inflammation. Nat Rev Immunol 16(1):51–67. https://doi.org/10.1038/nri.2015.4
Haworth O, Buckley CD (2015) Pathways involved in the resolution of inflammatory joint disease. Semin Immunol 27(3):194–199. https://doi.org/10.1016/j.smim.2015.04.002
Miyata J, Arita M (2015) Role of omega-3 fatty acids and their metabolites in asthma and allergic diseases. Allergol Int 64(1):27–34. https://doi.org/10.1016/j.alit.2014.08.003
Serhan CN, Chiang N, Dalli J (2015) The resolution code of acute inflammation: novel pro-resolving lipid mediators in resolution. Semin Immunol 27(3):200–215. https://doi.org/10.1016/j.smim.2015.03.004
Chiang N, Fredman G, Backhed F, Oh SF, Vickery T, Schmidt BA, Serhan CN (2012) Infection regulates pro-resolving mediators that lower antibiotic requirements. Nature 484(7395):524–528. https://doi.org/10.1038/nature11042
Colas RA, Shinohara M, Dalli J, Chiang N, Serhan CN (2014) Identification and signature profiles for pro-resolving and inflammatory lipid mediators in human tissue. Am J Physiol Cell Physiol 307(1):C39–C54. https://doi.org/10.1152/ajpcell.00024.2014
Dalli J, Colas RA, Arnardottir H, Serhan CN (2017) Vagal regulation of group 3 innate lymphoid cells and the Immunoresolvent PCTR1 controls infection resolution. Immunity 46(1):92–105. https://doi.org/10.1016/j.immuni.2016.12.009
Dalli J, Colas RA, Quintana C, Barragan-Bradford D, Hurwitz S, Levy BD, Choi AM, Serhan CN, Baron RM (2017) Human sepsis eicosanoid and proresolving lipid mediator temporal profiles: correlations with survival and clinical outcomes. Crit Care Med 45(1):58–68. https://doi.org/10.1097/CCM.0000000000002014
Dalli J, Kraft BD, Colas RA, Shinohara M, Fredenburgh LE, Hess DR, Chiang N, Welty-Wolf K, Choi AM, Piantadosi CA, Serhan CN (2015) The regulation of proresolving lipid mediator profiles in baboon pneumonia by inhaled carbon monoxide. Am J Respir Cell Mol Biol 53(3):314–325. https://doi.org/10.1165/rcmb.2014-0299OC
Dalli J, Serhan CN (2012) Specific lipid mediator signatures of human phagocytes: microparticles stimulate macrophage efferocytosis and pro-resolving mediators. Blood 120(15):e60–e72. https://doi.org/10.1182/blood-2012-04-423525
Weiss GA, Troxler H, Klinke G, Rogler D, Braegger C, Hersberger M (2013) High levels of anti-inflammatory and pro-resolving lipid mediators lipoxins and resolvins and declining docosahexaenoic acid levels in human milk during the first month of lactation. Lipids Health Dis 12:89. https://doi.org/10.1186/1476-511X-12-89
Serhan CN, Petasis NA (2011) Resolvins and protectins in inflammation resolution. Chem Rev 111(10):5922–5943. https://doi.org/10.1021/cr100396c
Knight J, Taylor GW, Wright P, Clare AS, Rowley AF (1999) Eicosanoid biosynthesis in an advanced deuterostomate invertebrate, the sea squirt (Ciona intestinalis). Biochim Biophys Acta 1436(3):467–478
Rathod KS, Kapil V, Velmurugan S, Khambata RS, Siddique U, Khan S, Van Eijl S, Gee LC, Bansal J, Pitrola K, Shaw C, D’Acquisto F, Colas RA, Marelli-Berg F, Dalli J, Ahluwalia A (2017) Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans. J Clin Invest 127(1):169–182. https://doi.org/10.1172/JCI89429
Titos E, Rius B, Lopez-Vicario C, Alcaraz-Quiles J, Garcia-Alonso V, Lopategi A, Dalli J, Lozano JJ, Arroyo V, Delgado S, Serhan CN, Claria J (2016) Signaling and immunoresolving actions of resolvin D1 in inflamed human visceral adipose tissue. J Immunol 197(8):3360–3370. https://doi.org/10.4049/jimmunol.1502522
Acknowledgments
This work was supported by funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (Grant number: 677542), a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (Grant number: 107613/Z/15/Z), and the Barts Charity (Grant number: MGU0343). CN Serhan is supported by the National Institutes of Health, USA Grant Number P01GM095467.
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Dalli, J., Colas, R.A., Walker, M.E., Serhan, C.N. (2018). Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_4
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DOI: https://doi.org/10.1007/978-1-4939-7592-1_4
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