Abstract
LC-MS/MS- and GC-MS-based targeted metabolomics is typically conducted by analyzing and quantifying a cascade of metabolites with methods specifically developed for the metabolite class. Here we describe an approach for the development of multi-residue analytical profiles, calibration standards, and internal standard solutions in support of a fast, simple, and low-cost plasma sample preparation that captures and quantitates a range of metabolite cascades.
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Acknowledgment
The authors would like to thank Ira J. Gray, Michael R. La Frano, and William R. Keyes for their technical support on the development and implementation of these assays over the past 10Â years. The methods reported in this chapter were developed as part of research projects funded by the United States Department of Agriculture (5306-51530-016-00D, 2032-51530-019-00-D, 2032-51530-022-00-D) and DK/NIDDK NIH HHS Grant U24 DK097154/United States
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1 Electronic Supplementary Material
Supplementary File 1
Interactive calibration standard preparation worksheet (XLSX 44 kb)
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Pedersen, T.L., Newman, J.W. (2018). Establishing and Performing Targeted Multi-residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples. In: Giera, M. (eds) Clinical Metabolomics. Methods in Molecular Biology, vol 1730. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7592-1_13
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DOI: https://doi.org/10.1007/978-1-4939-7592-1_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7591-4
Online ISBN: 978-1-4939-7592-1
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