Abstract
We describe a new method for obtaining user-friendly, robust pattern electroretinograms (PERG) simultaneously from each eye using asynchronous binocular stimulation and one-channel acquisition of signals recorded from a subcutaneous needle in the snout.
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References
Porciatti V (2015) Electrophysiological assessment of retinal ganglion cell function. Exp Eye Res 141:164–170. https://doi.org/10.1016/j.exer.2015.05.008
Porciatti V, Saleh M, Nagaraju M (2007) The pattern electroretinogram as a tool to monitor progressive retinal ganglion cell dysfunction in the DBA/2J mouse model of glaucoma. Invest Ophthalmol Vis Sci 48(2):745–751. https://doi.org/10.1167/iovs.06-0733
Chou TH, Porciatti V (2012) The bioelectric field of the pattern electroretinogram in the mouse. Invest Ophthalmol Vis Sci 53(13):8086–8092. https://doi.org/10.1167/iovs.12-10720
Chou TH (2011) Age-related structural and functional changes of the mouse eye: role of intraocular pressure and genotype. Biomedical engineering, vol 2011. University of Miami, Scholarly repository, Miami, FL, p 94
Chou TH, Bohorquez J, Toft-Nielsen J, Ozdamar O, Porciatti V (2014) Robust mouse pattern electroretinograms derived simultaneously from each eye using a common snout electrode. Invest Ophthalmol Vis Sci 55(4):2469–2475. https://doi.org/10.1167/iovs.14-13943
Schimmel H (1967) The (+) reference: accuracy of estimated mean components in average response studies. Science 157(3784):92–94
Acknowledgments
Grant support. NEI R43 EY023460 (JTN), NEI EY019077 (VP), NEI P30EY14801 (VP).
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Chou, TH., Toft-Nielsen, J., Porciatti, V. (2018). High-Throughput Binocular Pattern Electroretinograms in the Mouse. In: Jakobs, T. (eds) Glaucoma. Methods in Molecular Biology, vol 1695. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7407-8_6
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DOI: https://doi.org/10.1007/978-1-4939-7407-8_6
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7407-8
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