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In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

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Super-Resolution Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1663))

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

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Acknowledgments

The authors would like to thank members of the Wu, Zhuang, and Yin laboratories for extensive conversations and experience. This work was supported by grants from the NIH to the laboratories of C.-t W. (GM085169, DP1GM106412, RM1HG008525), X.Z. (R01GM105637), and P.Y. (1R01EB018659, 1-U01-MH106011), X.Z. is a Howard Hughes Medical Institute investigator. In addition, B.J.B. and A.N.B. were supported by Damon Runyon Postdoctoral Fellowships.

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Author notes

  1. Brian J. Beliveau, Alistair N. Boettiger, Guy Nir and Bogdan Bintu contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA

    Brian J. Beliveau, Guy Nir & C.-ting Wu

  2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA

    Brian J. Beliveau & Peng Yin

  3. Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA

    Brian J. Beliveau & Peng Yin

  4. Howard Hughes Medical Institute, Cambridge, MA, 02138, USA

    Alistair N. Boettiger, Bogdan Bintu & Xiaowei Zhuang

  5. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA

    Alistair N. Boettiger & Xiaowei Zhuang

  6. Department of Developmental Biology, Stanford University, Stanford, CA, 94305, USA

    Alistair N. Boettiger

  7. Department of Physics, Harvard University, Cambridge, MA, 02138, USA

    Bogdan Bintu & Xiaowei Zhuang

Authors
  1. Brian J. Beliveau
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  2. Alistair N. Boettiger
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  3. Guy Nir
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  4. Bogdan Bintu
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  5. Peng Yin
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  6. Xiaowei Zhuang
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  7. C.-ting Wu
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Corresponding authors

Correspondence to Peng Yin , Xiaowei Zhuang or C.-ting Wu .

Editor information

Editors and Affiliations

  1. High-Content Analysis of the Cell (HiCell) and Advanced Biological Screening Facility, BioQuant, Heidelberg University, Heidelberg, Germany

    Holger Erfle

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Beliveau, B.J. et al. (2017). In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT. In: Erfle, H. (eds) Super-Resolution Microscopy. Methods in Molecular Biology, vol 1663. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7265-4_19

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  • DOI: https://doi.org/10.1007/978-1-4939-7265-4_19

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7264-7

  • Online ISBN: 978-1-4939-7265-4

  • eBook Packages: Springer Protocols

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