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Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues

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Selenoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1661))

Abstract

Selenium and selenoproteins are important components of living organisms that play a role in different biological processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful analytical technique that has been employed to obtain distribution maps of selenium in biological tissues in a direct manner, as well as in selenoproteins, previously separated by their molecular masses and isoelectric points using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In this chapter, we present the protocols to perform LA-ICP-MS imaging experiments, allowing the distribution visualization and determination of selenium and/or selenoproteins in biological systems.

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Acknowledgments

A.S. thanks FAPESP (grant number 2015/13229-1) for financial support. E.C.S.C. thanks CNPq for her Ph.D. scholarship.

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Authors and Affiliations

  1. Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), P.O. Box 6154, Campinas, SP, 13083-970, Brazil

    Elisa Castañeda Santa Cruz & Alessandra Sussulini

  2. Konstanz, 78467, Germany

    J. Susanne Becker

  3. Zentralinstitut für Engineering, Elektronik und Analytik, Analytik (ZEA-3), Forschungszentrum Jülich, Jülich, Germany

    J. Sabine Becker

Authors
  1. Elisa Castañeda Santa Cruz
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  2. J. Susanne Becker
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  3. J. Sabine Becker
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  4. Alessandra Sussulini
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Corresponding authors

Correspondence to J. Sabine Becker or Alessandra Sussulini .

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Editors and Affiliations

  1. Centre International de Recherche en Infectiologie, CIRI, Lyon, France

    Laurent Chavatte

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Cruz, E.C.S., Susanne Becker, J., Sabine Becker, J., Sussulini, A. (2018). Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues. In: Chavatte, L. (eds) Selenoproteins. Methods in Molecular Biology, vol 1661. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7258-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7258-6_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7257-9

  • Online ISBN: 978-1-4939-7258-6

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