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BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance

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Wheat Rust Diseases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1659))

Abstract

Virus-induced gene silencing (VIGS) is a widely used reverse genetics tool to knock down genes in plants transiently without transformation. The assay has been successfully used to downregulate the transcript abundance of a target gene at almost any plant developmental stages in any tissues. Here, we describe the VIGS assay using a barley stripe mosaic virus (BSMV) for functional genomics analysis in wheat with the focus on genes involved in rust resistance.

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Author information

Authors and Affiliations

  1. Department of Plant Sciences and Plant Pathology, Montana State University, 307 Plant BioScience Bldg, P.O. Box 173150, Bozeman, MT, 59717, USA

    Li Huang

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  1. Li Huang
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Corresponding author

Correspondence to Li Huang .

Editor information

Editors and Affiliations

  1. Research School of Biology, Australian National University, Canberra, Aust Capital Terr, Australia

    Sambasivam Periyannan

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Huang, L. (2017). BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance. In: Periyannan, S. (eds) Wheat Rust Diseases. Methods in Molecular Biology, vol 1659. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7249-4_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7249-4_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7248-7

  • Online ISBN: 978-1-4939-7249-4

  • eBook Packages: Springer Protocols

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