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NMR and MS Methods for Metabolomics

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Drug Safety Evaluation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1641))

Abstract

Metabolomics, also often referred as “metabolic profiling,” is the systematic profiling of metabolites in biofluids or tissues of organisms and their temporal changes. In the last decade, metabolomics has become more and more popular in drug development, molecular medicine, and other biotechnology fields, since it profiles directly the phenotype and changes thereof in contrast to other “-omics” technologies. The increasing popularity of metabolomics has been possible only due to the enormous development in the technology and bioinformatics fields. In particular, the analytical technologies supporting metabolomics, i.e., NMR, UPLC-MS, and GC-MS, have evolved into sensitive and highly reproducible platforms allowing the determination of hundreds of metabolites in parallel. This chapter describes the best practices of metabolomics as seen today. All important steps of metabolic profiling in drug development and molecular medicine are described in great detail, starting from sample preparation to determining the measurement details of all analytical platforms, and finally to discussing the corresponding specific steps of data analysis.

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Authors and Affiliations

  1. Preclinical Safety, Sanofi R&D, Hattersheim, Germany

    Alexander Amberg

  2. Investigational Toxicology, Metabolic Profiling and Clinical Pathology, Bayer Pharma AG, Muellerstr. 178, Berlin, 13353, Germany

    Björn Riefke

  3. School of Life Sciences, Institute for Chemistry and Bioanalytics, University of Applied Sciences, Northwestern Switzerland, Muttenz, Switzerland

    Götz Schlotterbeck

  4. Roche Pharmaceutical Research and Early Development, Pharmaceutical Sciences, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland

    Alfred Ross

  5. Heythrop College UCL, Kensington Square, London W85HN, UK

    Hans Senn

  6. New Products and Medical, Near Patient Testing, Novartis, Basel, Switzerland

    Frank Dieterle

  7. Analytical Development 1, Bayer Pharma AG, Wupperal, 42096, Germany

    Matthias Keck

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  1. Alexander Amberg
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  2. Björn Riefke
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  3. Götz Schlotterbeck
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  4. Alfred Ross
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  5. Hans Senn
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  6. Frank Dieterle
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  7. Matthias Keck
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Correspondence to Björn Riefke .

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  1. Preclinical Safety, Sanofi, R&D, Vitry-sur-Seine, France

    Jean-Charles Gautier

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Amberg, A. et al. (2017). NMR and MS Methods for Metabolomics. In: Gautier, JC. (eds) Drug Safety Evaluation. Methods in Molecular Biology, vol 1641. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7172-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7172-5_13

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