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Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants

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Date Palm Biotechnology Protocols Volume I

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1637))

Abstract

A number of public and private laboratories are working on date palm micropropagation to meet the increasing worldwide demand for date palm planting material. A standardized direct organogenesis protocol exists for the production of date palm plantlets to maintain the genetic fidelity of regenerated plants. Organogenesis has the advantage of using low concentrations of plant growth regulators and avoiding the callus phase. In addition, direct regeneration of vegetative buds minimizes the risk of somaclonal variation among plant regenerants. However, in vitro multiplication cycles should be limited in duration by frequent renewal of plant material. This chapter describes a simple and routine organogenesis protocol for date palm multiplication using shoot tip explants.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, INRA, Marrakech Regional Center of Agricultural Research, P.O. Box 533, Marrakech, 40 000, Morocco

    Larbi Abahmane

Authors
  1. Larbi Abahmane
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Correspondence to Larbi Abahmane .

Editor information

Editors and Affiliations

  1. Department of Agricultural Biotechnology, King Faisal University, Al-Hassa, Saudi Arabia

    Jameel M. Al-Khayri

  2. Agricultural Sciences, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  3. Cincinnati, Ohio, USA

    Dennis V. Johnson

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Abahmane, L. (2017). Cultivar-Dependent Direct Organogenesis of Date Palm from Shoot Tip Explants. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume I. Methods in Molecular Biology, vol 1637. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7156-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7156-5_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7155-8

  • Online ISBN: 978-1-4939-7156-5

  • eBook Packages: Springer Protocols

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