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Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays

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Serum/Plasma Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1619))

Abstract

Protein microarrays are platforms for studying protein-protein interactions and identifying disease-related self-antigens/autoantigens, which elicit an immune response in a high-throughput format. Protein arrays have been extensively used over the past two decades for several clinical applications. By using this platform, serum containing autoantibodies against potential self-antigens can be screened on proteome-wide arrays, harboring a large repertoire of full-length human proteins. Identification of such autoantigens can help deducing early diagnostic, as well as, prognostic markers in case of malignancies, autoimmune disorders, and other systemic diseases. Here, we provide an overview of the protein microarray technology along with details of an established method to study autoantibody profiles from patient sera.

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Acknowledgments

This work has been supported from the grant BT/PR4599/BRB/10/1042/2012, 6242-P28/RGCB/PMD/DBT/SNJS/2015 Department of Biotechnology, India. The authors also acknowledge the help of Shaikh Khaja for editing the figures in this chapter.

Conflict of interest

The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India

    Shabarni Gupta, K. P. Manubhai, Shuvolina Mukherjee & Sanjeeva Srivastava

Authors
  1. Shabarni Gupta
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  2. K. P. Manubhai
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  3. Shuvolina Mukherjee
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  4. Sanjeeva Srivastava
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Corresponding author

Correspondence to Sanjeeva Srivastava .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    David W. Greening

  2. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    Richard J. Simpson

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Gupta, S., Manubhai, K.P., Mukherjee, S., Srivastava, S. (2017). Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7057-5_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7056-8

  • Online ISBN: 978-1-4939-7057-5

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