Abstract
3D in vitro culture systems may yield physiological outcomes that more closely approximate in vivo behavior. A number of fabrication techniques and hydrogel scaffold materials are available to researchers, but often their implementation is complex and seemingly prohibitive. Herein, we describe a simplistic and adaptable dual hydrogel photolithography method utilized to engineer advanced in vitro systems for studies of neuronal development and characterization.
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Acknowledgments
This work was supported in part by Tulane University, NIH R21-NS065374, an NSF CAREER Award to M.J.M. (CBET-1055990), and the DoD (W82XWH-12-1-0246).
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Lowry Curley, J., Moore, M.J. (2017). 3D Neural Culture in Dual Hydrogel Systems. In: Koledova, Z. (eds) 3D Cell Culture. Methods in Molecular Biology, vol 1612. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7021-6_17
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DOI: https://doi.org/10.1007/978-1-4939-7021-6_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7019-3
Online ISBN: 978-1-4939-7021-6
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